Human Recombinant ALDH1A1 protein, His Tag (V2LY-0526-LY1997)

Name: Human Recombinant ALDH1A1 protein, His Tag
SKU: V2LY-0526-LY1997
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ALDH1A1 protein, His Tag consist of Amino Acid: 2-501 and predicts a molecular mass of 41.5 kDa.

Molecule Mass

41.5 kDa

Verified

HPLC

Sequence

Amino Acid: 2-501

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Aldehyde Dehydrogenase 1 Family Member A1

Function

Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (PubMed:19296407, PubMed:26373694, PubMed:25450233).

Biological Process

Cellular aldehyde metabolic process Source: ProtInc
Ethanol oxidation Source: Reactome
Fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate Source: Reactome
Negative regulation of cold-induced thermogenesis Source: YuBioLab
Retinoid metabolic process Source: UniProtKB
Retinol metabolic process Source: UniProtKB-UniPathway

Cellular Location

Cytosol

PTM

The N-terminus is blocked most probably by acetylation.

Tunçer, S., Çamlica, R., & Yilmaz, I. (2020). ALDH1A1 (Aldehyde Dehydrogenase 1 family member A1). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

ALDH1A1 Matched Antibody Pair (062) (CAT#: APMAB-062LY)

Antibody Pairs

ALDH1A1 Matched Antibody Pair (062) (CAT#: APMAB-062LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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