Sign in or Register   Sign in or Register
  |  

Mouse Anti-ALDH1A1 Recombinant Antibody (B5) (CBMAB-0051CQ)

This product is a mouse antibody that recognizes ALDH1A1. The antibody mAb61293 can be used for immunoassay techniques such as: WB.
See all ALDH1A1 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human
Clone
B5
Antibody Isotype
IgG2a, κ
Application
ELISA, ICC, IF, IHC, WB, IP

Basic Information

Immunogen
Amino acids 105-137 near the N-terminus of ALDH1A1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:30-1:3,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldehyde Dehydrogenase 1 Family Member A1
Introduction
The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase (EC 1.2.1.3), an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase (ADH, see 103700). See also liver mitochondrial ALDH2 (100650), variation in which has been implicated in different responses to alcohol ingestion. ALDH1 is associated with a low Km for NAD, a high Km for acetaldehyde, and is strongly inactivated by disulfiram. ALDH2 is associated with a high Km for NAD, and low Km for acetaldehyde, and is insensitive to inhibition by disulfiram. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
Entrez Gene ID
UniProt ID
Alternative Names
Aldehyde Dehydrogenase 1 Family, Member A1; Retinaldehyde Dehydrogenase 1; EC 1.2.1.36; RALDH 1; ALDH-E1; RALDH1; ALHDII; ALDH1; PUMB1; ALDC; Epididymis Secretory Sperm Binding Protein Li 53e; Aldehyde Dehydrogenase Family 1 Member A1; Aldehyde Dehydrogenase, Liver Cytosolic; Aldehyde Dehydrogenase 1, Soluble; Aldehyde Dehydrogenase, Cytosolic; Epididymis Luminal Protein 12; Acetaldehyde Dehydrogenase 1; Epididymis Luminal Protein 9; ALDH Class 1; HEL-S-53e; EC 1.2.1; ALDH11; HEL-9; HEL12
Function
Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (PubMed:19296407, PubMed:26373694, PubMed:25450233).
Biological Process
Cellular aldehyde metabolic process Source: ProtInc
Ethanol oxidation Source: Reactome
Fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate Source: Reactome
Negative regulation of cold-induced thermogenesis Source: YuBioLab
Retinoid metabolic process Source: UniProtKB
Retinol metabolic process Source: UniProtKB-UniPathway
Cellular Location
Cytosol
PTM
The N-terminus is blocked most probably by acetylation.
Ask a question We look forward to hearing from you.
0 reviews or Q&As
Loading...
Have you used Mouse Anti-ALDH1A1 Recombinant Antibody (B5)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare