Mouse Anti-ALDH1A1 Recombinant Antibody (B5) (CBMAB-0051CQ)

Name: Mouse Anti-ALDH1A1 Recombinant Antibody (B5)
SKU: CBMAB-0051CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALDH1A1 Recombinant Antibody (B5) in WB

Altered expression of stemness-related proteins. ...View More

Yin, L., Li, Q., Mrdenovic, S., Chu, G. C. Y., Wu, B. J., Bu, H., ... & Chung, L. W. (2022). KRT13 promotes stemness and drives metastasis in breast cancer through a plakoglobin/c-Myc signaling pathway. Breast Cancer Research, 24(1), 1-13.

Mouse Anti-ALDH1A1 Recombinant Antibody (B5) in IHC

Simultaneously, the upregulations of KLF4 and ALDH1A1 and downregulation of LGR4 were validated at protein levels IHC. ...View More

Feng, Q., Li, S., Ma, H. M., Yang, W. T., & Zheng, P. S. (2021). LGR6 activates the Wnt/β-catenin signaling pathway and forms a β-catenin/TCF7L2/LGR6 feedback loop in LGR6high cervical cancer stem cells. Oncogene, 40(42), 6103-6114.

Mouse Anti-ALDH1A1 Recombinant Antibody (B5) in WB

Simultaneously, the upregulations of KLF4 and ALDH1A1 and downregulation of LGR4 were validated at protein levels WB. ...View More

Mouse Anti-ALDH1A1 Recombinant Antibody (B5) in IF

Colocalization analysis of VEGFR2 with ALDH1A1 (CSC marker) by immunofluorescence labeling of OPSCC. Immunohistochemical staining against VEGFR2 of a representative consecutive HPV-positive (a-f) and HPV-negative tumor section (g-l). (a,g) Overview and (b,h) details. A subpopulation of tumor cells with strong VEGFR2 immunoreactivity can be observed. Colocalization of VEGFR2 (d,j) with ALDH1A1 (f,l) was detected only in a subpopulation of tumor cells and at the subcellular level mainly in the cytoplasm (asterisks). The cells were distributed around the blood vessels (f,l). In HPV-positive OPSCC, some migrating cells were detected at the blood vessel wall and one cell is visible intravasally, while others were recognized in the fibrous tissue (f). Cell nuclei were stained with DRAQ5 (e,k). Scale bars: (a,g) 200 μm; (b,h) 50 μm; (c-f), (i-l) 20 μm. ...View More

Uzun, S., Korkmaz, Y., Wuerdemann, N., Arolt, C., Puladi, B., Siefer, O. G., ... & Huebbers, C. U. (2021). Comprehensive Analysis of VEGFR2 Expression in HPV-Positive and-Negative OPSCC Reveals Differing VEGFR2 Expression Patterns. Cancers, 13(20), 5221.

Mouse Anti-ALDH1A1 Recombinant Antibody (B5) in WB

Western blot analysis for proteins indicated on the left using protein lysates from KC6141 (KC) and T161 (KC Smad4Loss). ...View More

Nimmakayala, R. K., Rauth, S., Chirravuri Venkata, R., Marimuthu, S., Nallasamy, P., Vengoji, R., ... & Batra, S. K. (2021). PGC1α-mediated metabolic reprogramming drives the stemness of pancreatic precursor lesions. Clinical Cancer Research, 27(19), 5415-5429.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

ELISA, ICC, IF, IHC, WB, IP

Immunogen

Amino acids 105-137 near the N-terminus of ALDH1A1 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:30-1:3,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldehyde Dehydrogenase 1 Family Member A1

Introduction

The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase (EC 1.2.1.3), an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase (ADH, see 103700). See also liver mitochondrial ALDH2 (100650), variation in which has been implicated in different responses to alcohol ingestion. ALDH1 is associated with a low Km for NAD, a high Km for acetaldehyde, and is strongly inactivated by disulfiram. ALDH2 is associated with a high Km for NAD, and low Km for acetaldehyde, and is insensitive to inhibition by disulfiram. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.

Entrez Gene ID

216

UniProt ID

P00352

Alternative Names

Aldehyde Dehydrogenase 1 Family, Member A1; Retinaldehyde Dehydrogenase 1; EC 1.2.1.36; RALDH 1; ALDH-E1; RALDH1; ALHDII; ALDH1; PUMB1; ALDC; Epididymis Secretory Sperm Binding Protein Li 53e; Aldehyde Dehydrogenase Family 1 Member A1; Aldehyde Dehydrogenase, Liver Cytosolic; Aldehyde Dehydrogenase 1, Soluble; Aldehyde Dehydrogenase, Cytosolic; Epididymis Luminal Protein 12; Acetaldehyde Dehydrogenase 1; Epididymis Luminal Protein 9; ALDH Class 1; HEL-S-53e; EC 1.2.1; ALDH11; HEL-9; HEL12

Function

Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (PubMed:19296407, PubMed:26373694, PubMed:25450233).

Biological Process

Cellular aldehyde metabolic process Source: ProtInc
Ethanol oxidation Source: Reactome
Fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate Source: Reactome
Negative regulation of cold-induced thermogenesis Source: YuBioLab
Retinoid metabolic process Source: UniProtKB
Retinol metabolic process Source: UniProtKB-UniPathway

Cellular Location

Cytosol

PTM

The N-terminus is blocked most probably by acetylation.

Tunçer, S., Çamlica, R., & Yilmaz, I. (2020). ALDH1A1 (Aldehyde Dehydrogenase 1 family member A1). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ALDH1A1 Matched Antibody Pair (062) (CAT#: APMAB-062LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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