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Mouse Anti-ALDH1A1 Recombinant Antibody (A6) (CBMAB-A2241-YC)

Provided herein is a Mouse monoclonal antibody against Human Aldehyde Dehydrogenase 1 Family Member A1. The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all ALDH1A1 antibodies
Published Data
Mouse Anti-ALDH1A1 Recombinant Antibody (A6) in WB
Western blot analysis of ALDH1, Oct-4, SOX2, and Nanog in ALDH1-positive cells and ALDH1-negative cells. b-tubulin was used as a control. ...View More
Zhang, S., & Zhang, X. (2019). Shrimp miRNA suppresses the stemness of human cancer stem cells via the PIN1 pathway. The FASEB Journal, 33(10), 10767-10779.

Summary

Host Animal
Mouse
Specificity
Human
Clone
A6
Antibody Isotype
IgG1, κ
Application
ELISA, ICC, IF, WB

Basic Information

Immunogen
Amino acids 3-21 at the N-terminus of ALDH1A1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:30-1:3,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldehyde Dehydrogenase 1 Family Member A1
Introduction
ALDH1A1 belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitocho
Entrez Gene ID
216
UniProt ID
P00352
Alternative Names
Aldehyde Dehydrogenase 1 Family Member A1; Retinaldehyde Dehydrogenase 1; EC 1.2.1.36; RALDH 1; ALDH-E1; RALDH1; ALHDII; ALDH1; PUMB1; ALDC; Epididymis Secretory Sperm Binding Protein Li 53e; Aldehyde Dehydrogenase 1 Family, Member A1; Aldehyde Dehydrogen
Function
Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (PubMed:19296407, PubMed:26373694, PubMed:25450233).
Biological Process
Cellular aldehyde metabolic process Source: ProtInc
Ethanol oxidation Source: Reactome
Fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate Source: Reactome
Negative regulation of cold-induced thermogenesis Source: YuBioLab
Retinoid metabolic process Source: UniProtKB
Retinol metabolic process Source: UniProtKB-UniPathway
Cellular Location
Cytosol
PTM
The N-terminus is blocked most probably by acetylation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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