Human Recombinant ART1 protein, His Tag (V2LY-0526-LY2177)

Name: Human Recombinant ART1 protein, His Tag
SKU: V2LY-0526-LY2177
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ART1 protein, His Tag consist of Amino Acid: 1-294 and predicts a molecular mass of 31.55 kDa.

Molecule Mass

31.55 kDa

Sequence

Amino Acid: 1-294

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile MES, NaCl, ZnCl2, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ADP-Ribosyltransferase 1

Function

Has ADP-ribosyltransferase activity toward GLP1R.

Biological Process

Peptidyl-arginine ADP-ribosylation Source: GO_Central
Protein ADP-ribosylation Source: ProtInc

Cellular Location

Sarcoplasmic reticulum membrane

Mukherjee, S., Wennerberg, E., Hung, C., Saadallah, N., Kariyawasam, S., Hussein, M. K., ... & Stiles, B. M. (2020). A05 ART1, a Mono-ADP-Ribosyltransferase, Regulates Tumor-Infiltrating CD8+ T Cells and Is Highly Expressed in EGFR Mutated Lung Cancers. Journal of Thoracic Oncology, 15(2), S13.

Mukherjee, S., Wennerberg, E., Hung, C., Saadallah, N., Kariyawasam, S., Agrusa, C., ... & Stiles, B. (2020). Art1, an extracellular mono-ADP-ribosyltransferase, is upregulated in response to cellular stress and promotes lung cancer growth.

Chen, W., Yang, Y., Duan, J., Li, M., Tang, Y., Zhang, S., ... & Wang, Y. (2020). High Norepinephrine Status Induced Growth of Colorectal Cancer With Type 2 Diabetes Mellitus Benefited From ADP-Ribosyltransferase 1.

Zhou, L., Zhan, M. L., Tang, Y., Xiao, M., Li, M., Li, Q. S., ... & Wang, Y. L. (2018). Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions. International journal of oncology, 53(4), 1613-1624.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant ART1 protein, His Tag (CAT#: V2LY-0526-LY1375)

Human ART1 ELISA Kit (CAT#: V2LY-0626-LY4633)

Related Products

Mouse Anti-ART1 Recombinant Antibody (6E2) (CAT#: CBMAB-A3682-YC)

Cynomolgus Recombinant ART1 protein, His Tag (CAT#: V2LY-0526-LY1375)

Human ART1 ELISA Kit (CAT#: V2LY-0626-LY4633)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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