Human Recombinant Aspartate Aminotransferase protein, His Tag (V2LY-0526-LY2187)

Name: Human Recombinant Aspartate Aminotransferase protein, His Tag
SKU: V2LY-0526-LY2187
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant Aspartate Aminotransferase protein, His Tag consist of Amino Acid: 1-413 and predicts a molecular mass of 48.1 kDa.

Molecule Mass

48.1 kDa

Verified

HPLC

Sequence

Amino Acid: 1-413

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Glutamic-Oxaloacetic Transaminase 1

Function

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Biological Process

2-oxoglutarate metabolic process Source: UniProtKB
Aspartate biosynthetic process Source: GO_Central
Aspartate catabolic process Source: UniProtKB
Aspartate metabolic process Source: UniProtKB
Cellular response to insulin stimulus Source: UniProtKB
Cellular response to mechanical stimulus Source: Ensembl
Fatty acid homeostasis Source: Ensembl
Glutamate catabolic process to 2-oxoglutarate Source: Ensembl
Glutamate catabolic process to aspartate Source: Ensembl
Glutamate metabolic process Source: UniProtKB
Glycerol biosynthetic process Source: UniProtKB
Negative regulation of collagen biosynthetic process Source: Ensembl
Negative regulation of cytosolic calcium ion concentration Source: Ensembl
Negative regulation of mitochondrial depolarization Source: Ensembl
Notch signaling pathway Source: Ensembl
Oxaloacetate metabolic process Source: Ensembl
Positive regulation of transforming growth factor beta receptor signaling pathway Source: Ensembl
Response to cadmium ion Source: Ensembl
Response to carbohydrate Source: Ensembl
Response to glucocorticoid Source: UniProtKB
Response to immobilization stress Source: Ensembl
Response to transition metal nanoparticle Source: Ensembl
Transdifferentiation Source: Ensembl

Cellular Location

Cytoplasm

Song, Z., Yang, Y., Wu, Y., Zheng, M., Sun, D., Li, H., & Chen, L. (2022). Glutamic oxaloacetic transaminase 1 as a potential target in human cancer. European Journal of Pharmacology, 174754.

Yang, Y., Cheng, Z., Zhang, W., Hei, W., Lu, C., Cai, C., ... & Li, B. (2022). Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content. Animal Bioscience, 35(2), 155.

Deng, Y., Lai, W., Yu, L., Zhang, W., & Ding, Y. (2022). miR-2115-3p inhibits ferroptosis by downregulating the expression of glutamic-oxaloacetic transaminase in preeclampsia. Placenta, 129, 94-103.

Cheng, Z., Dai, Y., Zeng, T., Liu, Y., Cui, L., Qian, T., ... & Fu, L. (2020). Upregulation of glutamic-oxaloacetic transaminase 1 predicts poor prognosis in acute myeloid leukemia. Frontiers in oncology, 10, 379.

Weißhaar, N. K. (2020). The malate aspartate shuttle in T cell metabolic fitness: Glutamic oxaloacetic transaminase 1 antagonizes T cell exhaustion (Doctoral dissertation).

Wang, Q., Zhang, Q., Luan, S., Yang, K., Zheng, M., Li, K., ... & Li, H. (2019). Adapalene inhibits ovarian cancer ES-2 cells growth by targeting glutamic-oxaloacetic transaminase 1. Bioorganic Chemistry, 93, 103315.

Zhang, K., Wu, L., Zhang, P., Luo, M., Du, J., Gao, T., ... & Yang, Y. (2018). miR‐9 regulates ferroptosis by targeting glutamic‐oxaloacetic transaminase GOT1 in melanoma. Molecular carcinogenesis, 57(11), 1566-1576.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Got1 ELISA Kit (CAT#: V2LY-0626-LY1423)

Pig GOT1 ELISA Kit (CAT#: V2LY-0626-LY929)

GOT1 Matched Antibody Pair (493) (CAT#: APMAB-493LY)

Antibody Pairs

GOT1 Matched Antibody Pair (493) (CAT#: APMAB-493LY)

Related Products

Pig GOT1 ELISA Kit (CAT#: V2LY-0626-LY929)

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Mouse Anti-GOT1 Recombinant Antibody (4G4) (CAT#: V2LY-0226-LY437)

GOT1 Matched Antibody Pair (493) (CAT#: APMAB-493LY)

Mouse Anti-GOT1 Recombinant Antibody (CBWJC-3731) (CAT#: V2LY-0226-LY444)

Mouse Anti-GOT1 Recombinant Antibody (3G11) (CAT#: V2LY-0226-LY441)

Mouse Anti-GOT1 Recombinant Antibody (CBLG1-1542) (CAT#: V2LY-0226-LY439)

Mouse Anti-GOT1 Recombinant Antibody (1342) (CAT#: CBMAB-A4427-YC)

Mouse Anti-GOT1 Recombinant Antibody (CF188) (CAT#: CBMAB-FT303LY)

Isotype control

For research use only. Not intended for any clinical use.

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