Human Recombinant ATF6 protein, GST Tag (V2LY-0526-LY2192)

Name: Human Recombinant ATF6 protein, GST Tag
SKU: V2LY-0526-LY2192
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant ATF6 protein, GST Tag consist of Amino Acid: 1-373 and predicts a molecular mass of 69 kDa.

Molecule Mass

69 kDa

Sequence

Amino Acid: 1-373

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

MOPS, NaCl, Glutathione, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Activating Transcription Factor 6

Function

Cyclic AMP-dependent transcription factor ATF-6 alpha: Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane (PubMed:10564271, PubMed:11158310, PubMed:11779464).
Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR) (PubMed:10564271, PubMed:11158310, PubMed:11779464).
Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Transcription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR (PubMed:10564271, PubMed:11163209, PubMed:11158310, PubMed:11779464).
Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3') (PubMed:10564271, PubMed:11158310, PubMed:11779464).
Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor (PubMed:10564271, PubMed:11158310, PubMed:11779464).
May play a role in foveal development and cone function in the retina (PubMed:26029869).

Biological Process

ATF6-mediated unfolded protein response Source: ParkinsonsUK-UCL
Endoplasmic reticulum unfolded protein response Source: GO_Central
Eye development Source: UniProtKB
Positive regulation of apoptotic process Source: ParkinsonsUK-UCL
Positive regulation of ATF6-mediated unfolded protein response Source: UniProtKB
Positive regulation of autophagy Source: MGI
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Protein folding Source: ProtInc
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc
Visual perception Source: UniProtKB

Cellular Location

Golgi apparatus membrane; Endoplasmic reticulum membrane. Translocates from the endoplasmic reticulum to the Golgi, where it is processed.
Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus (PubMed:10564271, PubMed:12782636). THBS4 promotes its nuclear shuttling (By similarity).

Involvement in disease

Achromatopsia 7 (ACHM7): A form of achromatopsia, an ocular stationary disorder due to the absence of functioning cone photoreceptors in the retina. It is characterized by total colorblindness, low visual acuity, photophobia and nystagmus.

Topology

Cytoplasmic: 1-377 aa
Helical: 378-398 aa
Lumenal: 399-670 aa

PTM

During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).

Shen, Y., Li, R., Yu, S., Zhao, Q., Wang, Z., Sheng, H., & Yang, W. (2021). Activation of the ATF6 (Activating Transcription Factor 6) Signaling Pathway in Neurons Improves Outcome After Cardiac Arrest in Mice. Journal of the American Heart Association, e020216.

Ji, Z., Zhou, Z. L., Hao, Q., Zhao, L., Cui, C., Huang, S. B., ... & Shen, Y. Q. (2021). Activating Transcription Factor 6 Contributes to Functional Recovery After Spinal Cord Injury in Adult Zebrafish. Journal of Molecular Neuroscience, 71(4), 734-745.

Cheng, N., Shi, J. H., Jin, Y., Shi, Y. B., Liu, X. D., Zhang, H. P., ... & Zhang, S. J. (2021). Pharmacological Activating Transcription Factor 6 Activation Is Beneficial for Liver Retrieval With ex vivo Normothermic Mechanical Perfusion From Cardiac Dead Donor Rats. Frontiers in Surgery, 8, 212.

Tao, J., Chen, H., Li, X., & Wang, J. (2021). The role of activating transcription factor 6 in hydroxycamptothecin-induced fibroblast autophagy and apoptosis. Journal of Orthopaedic Surgery and Research, 16(1), 1-8.

Stengel, S. T., Fazio, A., Lipinski, S., Jahn, M. T., Aden, K., Ito, G., ... & Rosenstiel, P. (2020). Activating transcription factor 6 mediates inflammatory signals in intestinal epithelial cells upon endoplasmic reticulum stress. Gastroenterology, 159(4), 1357-1374.

Liu, F., Chang, L., & Hu, J. (2020). Activating transcription factor 6 regulated cell growth, migration and inhibiteds cell apoptosis and autophagy via MAPK pathway in cervical cancer. Journal of reproductive immunology, 139, 103120.

Du, Y., Liu, X., Zhu, X., Liu, Y., Wang, X., & Wu, X. (2020). Activating transcription factor 6 reduces Aβ1–42 and restores memory in Alzheimer’s disease model mice. International Journal of Neuroscience, 130(10), 1015-1023.

Shen, T., Li, Y., Chen, Z., Liang, S., Qiu, Y., Zhu, L., ... & Qiu, L. (2020). Activating transcription factor 6 (ATF6) negatively regulates Polo-like kinase 4 expression via recruiting C/EBPβ to the upstream-promoter during ER stress. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1863(2), 194488.

Liu, S., Wang, W., Ge, W., Lv, X., Han, Z., Li, Y., ... & Song, L. (2020). An activating transcription factor 6 beta (ATF6β) regulates apoptosis of hemocyte during immune response in Crassostrea gigas. Fish & shellfish immunology, 99, 442-451.

Jegal, K. H., Park, S. M., Cho, S. S., Byun, S. H., Ku, S. K., Kim, S. C., ... & Cho, I. J. (2017). Activating transcription factor 6-dependent sestrin 2 induction ameliorates ER stress-mediated liver injury. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1864(7), 1295-1307.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

ATF6 Matched Antibody Pair (115) (CAT#: APMAB-115LY)

Antibody Pairs

ATF6 Matched Antibody Pair (115) (CAT#: APMAB-115LY)

Related Products

ATF6 Matched Antibody Pair (115) (CAT#: APMAB-115LY)

Mouse Anti-ATF6 Recombinant Antibody (3D5) (CAT#: CBMAB-A0569-LY)

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For research use only. Not intended for any clinical use.

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