Human Recombinant ATP1B2 protein, His Tag (V2LY-0526-LY2201)

Name: Human Recombinant ATP1B2 protein, His Tag
SKU: V2LY-0526-LY2201
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ATP1B2 protein, His Tag consist of Amino Acid: 68-290 and predicts a molecular mass of 27.7 kDa.

Molecule Mass

27.7 kDa

Sequence

Amino Acid: 68-290

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ATPase, Na+/K+ transporting, beta 2 polypeptide

Function

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.

Biological Process

Cell communication by electrical coupling involved in cardiac conduction Source: BHF-UCL
Cell-substrate adhesion Source: ARUK-UCL
Cellular potassium ion homeostasis Source: BHF-UCL
Cellular sodium ion homeostasis Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Lateral ventricle development Source: ARUK-UCL
Leukocyte migration Source: Reactome
Membrane repolarization Source: BHF-UCL
Motor behavior Source: ARUK-UCL
Negative regulation of glial cell migration Source: ARUK-UCL
Neuronal-glial interaction involved in hindbrain glial-mediated radial cell migration Source: ARUK-UCL
Photoreceptor cell maintenance Source: ARUK-UCL
Plasma membrane bounded cell projection organization Source: ARUK-UCL
Positive regulation of ATPase activity Source: BHF-UCL
Positive regulation of neuron projection development Source: ARUK-UCL
Positive regulation of potassium ion import across plasma membrane Source: BHF-UCL
Positive regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
Positive regulation of sodium ion export across plasma membrane Source: BHF-UCL
Potassium ion import across plasma membrane Source: BHF-UCL
Protein stabilization Source: BHF-UCL
Regulation of cardiac conduction Source: Reactome
Retina homeostasis Source: ARUK-UCL
Sodium ion export across plasma membrane Source: BHF-UCL
Third ventricle development Source: ARUK-UCL
Transport across blood-brain barrier Source: ARUK-UCL

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-39 aa
Helical: 40-67 aa
Extracellular: 68-290 aa

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-ATP1B2 Recombinant Antibody (4E3) (CAT#: CBMAB-1166-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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