Human Recombinant CALCR protein, ECD, hFc Tag (V2LY-0526-LY2510)

Name: Human Recombinant CALCR protein, ECD, hFc Tag
SKU: V2LY-0526-LY2510
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CALCR protein, ECD, hFc Tag consist of Amino Acid: 1-153 and predicts a molecular mass of 42.1 kDa.

Molecule Mass

42.1 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-153

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Calcitonin Receptor

Function

This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.
Isoform 2:
Receptor for calcitonin but is unable to couple to G proteins and activate adenylyl cyclase (PubMed:7476993).
Does not undergo receptor internalization following ligand binding (PubMed:7476993).

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Amylin receptor signaling pathway Source: UniProtKB
Cell surface receptor signaling pathway Source: InterPro
Cross-receptor inhibition within G protein-coupled receptor heterodimer Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Positive regulation of adenylate cyclase activity Source: UniProtKB
Positive regulation of calcium ion import across plasma membrane Source: ARUK-UCL
Positive regulation of cell death Source: ARUK-UCL
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: ARUK-UCL
Positive regulation of gene expression Source: ARUK-UCL
Positive regulation of peptidyl-serine phosphorylation Source: ARUK-UCL
Positive regulation of protein kinase A signaling Source: ARUK-UCL
Positive regulation of protein kinase B signaling Source: ARUK-UCL
Response to amyloid-beta Source: ARUK-UCL
Response to glucocorticoid Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 25-152 aa
Helical: 153-173 aa
Cytoplasmic: 174-186 aa
Helical: 187-207 aa
Extracellular: 208-233 aa
Helical: 234-254 aa
Cytoplasmic: 255-258 aa
Helical: 259-279 aa
Extracellular: 280-296 aa
Helical: 297-317 aa
Cytoplasmic: 318-340 aa
Helical: 341-361 aa
Extracellular: 362-373 aa
Helical: 374-394 aa
Cytoplasmic: 395-474 aa

Zhang, L., Kubota, M., Nakamura, A., Kaji, T., Seno, S., Uezumi, A., ... & Fukada, S. I. (2021). Dlk1 regulates quiescence in calcitonin receptor‐mutant muscle stem cells. Stem Cells, 39(3), 306-317.

Gonzalez, I. E., Ramirez-Matias, J., Lu, C., Pan, W., Zhu, A., Myers Jr, M. G., & Olson, D. P. (2021). Paraventricular Calcitonin Receptor–Expressing Neurons Modulate Energy Homeostasis in Male Mice. Endocrinology, 162(6), bqab072.

Yoshihara, C., Tokita, K., Maruyama, T., Kaneko, M., Tsuneoka, Y., Fukumitsu, K., ... & Kuroda, K. O. (2021). Calcitonin receptor signaling in the medial preoptic area enables risk-taking maternal care. Cell Reports, 35(9), 109204.

Cheng, W., Gonzalez, I., Pan, W., Tsang, A. H., Adams, J., Ndoka, E., ... & Myers Jr, M. G. (2020). Calcitonin receptor neurons in the mouse nucleus tractus solitarius control energy balance via the non-aversive suppression of feeding. Cell metabolism, 31(2), 301-312.

Ikemoto‐Uezumi, M., Uezumi, A., Zhang, L., Zhou, H., Hashimoto, N., Okamura, K., ... & Fukada, S. I. (2019). Reduced expression of calcitonin receptor is closely associated with age‐related loss of the muscle stem cell pool. JCSM Rapid Communications, 2(1), 1-13.

Ieda, N., Kawai, N., Ishii, H., Ihara, K., Inoue, N., Uenoyama, Y., & Tsukamura, H. (2018). Co‐expression of the calcitonin receptor gene in the hypothalamic kisspeptin neurons in female rats. Reproductive medicine and biology, 17(2), 164-172.

Pan, W., Adams, J. M., Allison, M. B., Patterson, C., Flak, J. N., Jones, J., ... & Myers Jr, M. G. (2018). Essential Role for Hypothalamic Calcitonin Receptor‒Expressing Neurons in the Control of Food Intake by Leptin. Endocrinology, 159(4), 1860-1872.

Pal, J., Patil, V., Kumar, A., Kaur, K., Sarkar, C., & Somasundaram, K. (2018). Loss-of-function mutations in Calcitonin receptor (CALCR) identify highly aggressive glioblastoma with poor outcome. Clinical Cancer Research, 24(6), 1448-1458.

Mitra, P., Guha, M., Ghosh, S., Mukherjee, S., Bankura, B., Pal, D. K., ... & Das, M. (2017). Association of calcitonin receptor gene (CALCR) polymorphism with kidney stone disease in the population of West Bengal, India. Gene, 622, 23-28.

Yuan, J., Gilbert, E. R., & Cline, M. A. (2017). The central anorexigenic mechanism of amylin in Japanese quail (Coturnix japonica) involves pro-opiomelanocortin, calcitonin receptor, and the arcuate nucleus of the hypothalamus. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 210, 28-34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CALCR protein, hFc Tag (CAT#: V2LY-0526-LY2511)

CALCR Matched Antibody Pair (168) (CAT#: APMAB-168LY)

Antibody Pairs

CALCR Matched Antibody Pair (168) (CAT#: APMAB-168LY)

Related Products

Human Recombinant CALCR protein, hFc Tag (CAT#: V2LY-0526-LY2511)

CALCR Matched Antibody Pair (168) (CAT#: APMAB-168LY)

Rabbit Anti-CALCR Recombinant Antibody (CBFYC-0742) (CAT#: CBMAB-C0797-FY)

Mouse Anti-CALCR Recombinant Antibody (2F7) (CAT#: CBMAB-C5113-LY)

Mouse Anti-CALCR Recombinant Antibody (CBLNC-135) (CAT#: CBMAB-1287-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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