Human Recombinant CAPG protein (V2LY-0526-LY2547)

Name: Human Recombinant CAPG protein
SKU: V2LY-0526-LY2547
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Protein Construction

This product is Human Recombinant CAPG protein consist of Amino Acid: 1-348 and predicts a molecular mass of 38.5 kDa.

Molecule Mass

38.5 kDa

Sequence

Amino Acid: 1-348

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Capping Actin Protein, Gelsolin Like

Function

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.

Biological Process

Actin filament severing Source: GO_Central
Actin polymerization or depolymerization Source: GO_Central
Barbed-end actin filament capping Source: GO_Central
Cell projection assembly Source: GO_Central
Central nervous system development Source: GO_Central
Protein-containing complex assembly Source: ProtInc

Cellular Location

Nucleus; Cytoplasm; Melanosome; Lamellipodium; Ruffle. In macrophages, may be predominantly cytoplasmic. Nuclear localization was observed in fibroblasts. In macrophages, present at the membrane-cytoplasm interface. In activated macrophages, concentrated in the ruffles of the leading lamellipodia.

PTM

The N-terminus is blocked.

Prescher, N., Hänsch, S., Knobbe-Thomsen, C. B., Stühler, K., & Poschmann, G. (2021). The migration behavior of human glioblastoma cells is influenced by the redox-sensitive human macrophage capping protein CAPG. Free Radical Biology and Medicine, 167, 81-93.

Bahrami, S., Gheysarzadeh, A., Sotoudeh, M., Bandehpour, M., Zali, H., Hedayati, M., & Basiri, A. (2020). The Association Between Gelsolin-like Actin-capping Protein (CapG) Overexpression and Bladder Cancer Prognosis. Urology journal, 5664-5664.

Wei, J., Feng, L., & Wu, L. (2020). Integrated analysis identified CAPG as a prognosis factor correlated with immune infiltrates in lower‐grade glioma. Clinical and translational medicine, 10(2).

Zhaojie, L., Yuchen, L., Miao, C., Yacun, C., Shayi, W., Anbang, H., ... & Xinyuan, G. (2019). Gelsolin-like actin-capping protein has prognostic value and promotes tumorigenesis and epithelial-mesenchymal transition via the Hippo signaling pathway in human bladder cancer. Therapeutic advances in medical oncology, 11, 1758835919841235.

Lang, Z., Chen, Y., Zhu, H., Sun, Y., Zhang, H., Huang, J., & Zou, Z. (2019). Prognostic and clinicopathological significance of CapG in various cancers: Evidence from a meta-analysis. Pathology-Research and Practice, 215(12), 152683.

Yun, D. P., Wang, Y. Q., Meng, D. L., Ji, Y. Y., Chen, J. X., Chen, H. Y., & Lu, D. R. (2018). Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma. Oncology reports, 39(3), 1011-1022.

Tsai, T. J., Chao, W. Y., Chen, C. C., Chen, Y. J., Lin, C. Y., & Lee, Y. R. (2018). Gelsolin-like Actin-capping Protein (CapG) Overexpression in the Cytoplasm of Human Hepatocellular Carcinoma, Associated with Cellular Invasion, Migration and Tumor Prognosis. Anticancer research, 38(7), 3943-3950.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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