Human Recombinant CBG protein, His Tag (V2LY-0526-LY3345)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant CBG protein, His Tag consist of Amino Acid: 1-405 and predicts a molecular mass of 44 kDa.
Molecule Mass
44 kDa
Verified
HPLC
Sequence
Amino Acid: 1-405
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥98% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Serpin Family A Member 6
Function
Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
Biological Process
Biological Process glucocorticoid metabolic processIEA:Ensembl
Biological Process negative regulation of endopeptidase activityManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Secreted
Involvement in disease
Corticosteroid-binding globulin deficiency (CBG deficiency):
Extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue.
PTM
N-glycosylated; binds 5 oligosaccharide chains.
Glycosylation in position Asn-260 is needed for steroid binding.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CBG protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY3344)

Mouse Recombinant SERPINA6 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY8152)

Mouse Recombinant SERPINA6 protein, His Tag (CAT#: V2LY-0526-LY8153)

SERPINA6 Matched Antibody Pair (1064) (CAT#: APMAB-1064LY)

SERPINA6 Matched Antibody Pair (1065) (CAT#: APMAB-1065LY)

Antibody Pairs

SERPINA6 Matched Antibody Pair (1064) (CAT#: APMAB-1064LY)

SERPINA6 Matched Antibody Pair (1065) (CAT#: APMAB-1065LY)

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Mouse Anti-SERPINA6 Recombinant Antibody (CBXS-1984) (CAT#: CBMAB-S4753-CQ)

SERPINA6 Matched Antibody Pair (1064) (CAT#: APMAB-1064LY)

SERPINA6 Matched Antibody Pair (1065) (CAT#: APMAB-1065LY)

Mouse Anti-SERPINA6 Recombinant Antibody (CAP337) (CAT#: CBMAB-AP836LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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