Human Recombinant CCL23 protein, His Tag-1 (V2LY-0526-LY5632)

Name: Human Recombinant CCL23 protein, His Tag-1
SKU: V2LY-0526-LY5632
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Yeast

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CCL23 protein, His Tag consist of Amino Acid: 46-120 and predicts a molecular mass of 9.9 kDa.

Molecule Mass

9.9 kDa

Sequence

Amino Acid: 46-120

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

C-C Motif Chemokine Ligand 23

Function

Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.

Biological Process

Cell-cell signaling Source: ProtInc
Cellular calcium ion homeostasis Source: ProtInc
Cellular response to interferon-gamma Source: GO_Central
Cellular response to interleukin-1 Source: GO_Central
Cellular response to tumor necrosis factor Source: GO_Central
Chemokine-mediated signaling pathway Source: GO_Central
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: GO_Central
Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Lymphocyte chemotaxis Source: GO_Central
Monocyte chemotaxis Source: GO_Central
Negative regulation of C-C chemokine binding Source: UniProtKB
Negative regulation of cell population proliferation Source: ProtInc
Neutrophil chemotaxis Source: GO_Central
Positive regulation of ERK1 and ERK2 cascade Source: GO_Central
Positive regulation of GTPase activity Source: GO_Central
Signal transduction Source: ProtInc

Cellular Location

Secreted

Lu, J., Yang, Y., Yu, P., Tao, H., Lu, X., Wang, L., ... & Chen, C. (2019). Bioinformatics analysis and significance of expression of CC chemokine ligand 23 (CCL23) in hepatocellular carcinoma. Xi bao yu fen zi mian yi xue za zhi= Chinese journal of cellular and molecular immunology, 35(10), 903-909.

Lim, W., Bae, H., Bazer, F. W., & Song, G. (2018). C‐C motif chemokine ligand 23 abolishes ER stress‐and LPS‐induced reduction in proliferation of bovine endometrial epithelial cells. Journal of cellular physiology, 233(4), 3529-3539.

Jeong, W., Bae, H., Lim, W., Bazer, F. W., & Song, G. (2017). Differential expression and functional roles of chemokine (CC motif) ligand 23 and its receptor chemokine (CC motif) receptor type 1 in the uterine endometrium during early pregnancy in pigs. Developmental & Comparative Immunology, 76, 316-325.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CCL23 protein (CAT#: V2LY-0526-LY5630)

Human Recombinant CCL23 protein, His Tag (CAT#: V2LY-0526-LY5631)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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