Human Recombinant CD109 protein, His Tag (V2LY-0526-LY2656)

Name: Human Recombinant CD109 protein, His Tag
SKU: V2LY-0526-LY2656
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD109 protein, His Tag consist of Amino Acid: 1-1269 and predicts a molecular mass of 141.23 kDa.

Molecule Mass

141.23 kDa

Verified

HPLC

Sequence

Amino Acid: 1-1269

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD109 Molecule

Function

Modulates negatively TGFB1 signaling in keratinocytes.

Biological Process

Hair follicle development Source: Ensembl
Negative regulation of keratinocyte proliferation Source: Ensembl
Negative regulation of protein phosphorylation Source: Ensembl
Negative regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
Negative regulation of wound healing Source: UniProtKB
Osteoclast fusion Source: Ensembl
Platelet degranulation Source: Reactome
Regulation of keratinocyte differentiation Source: Ensembl

Cellular Location

Cell membrane

PTM

N-glycosylated.
2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.

Merle, N. S., Kolev, M., Rahman, J., West, E., Yan, B., Kazemian, M., ... & Kemper, C. (2021). The C3-like molecule CD109 controls Th1 versus Th17 induction in CD4+ T cells.

Lee, K. Y., Kuo, T. C., Chou, C. M., Hsu, W. J., Lee, W. C., Dai, J. Z., ... & Lin, C. W. (2021). Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling. Cells, 10(1), 28.

Li, C., Cho, H. J., Yamashita, D., Abdelrashid, M., Chen, Q., Bastola, S., ... & Nakano, I. (2020). Tumor edge-to-core transition promotes malignancy in primary-to-recurrent glioblastoma progression in a PLAGL1/CD109-mediated mechanism. Neuro-oncology advances, 2(1), vdaa163.

Lee, K. Y., Shueng, P. W., Chou, C. M., Lin, B. X., Lin, M. H., Kuo, D. Y., ... & Lin, C. W. (2020). Elevation of CD109 promotes metastasis and drug resistance in lung cancer via activation of EGFR‐AKT‐mTOR signaling. Cancer science, 111(5), 1652.

Hatsuzawa, Y., Yamaguchi, K., Takanashi, T., Sato, I., Tamai, K., Mochizuki, M., ... & Sugamura, K. (2020). CD109 promotes the tumorigenic ability and metastatic motility of pancreatic ductal adenocarcinoma cells. Pancreatology, 20(3), 493-500.

Zhou, S., da Silva, S. D., Siegel, P. M., & Philip, A. (2019). CD109 acts as a gatekeeper of the epithelial trait by suppressing epithelial to mesenchymal transition in squamous cell carcinoma cells in vitro. Scientific reports, 9(1), 1-17.

Mii, S., Enomoto, A., Shiraki, Y., Taki, T., Murakumo, Y., & Takahashi, M. (2019). CD109: a multifunctional GPI‐anchored protein with key roles in tumor progression and physiological homeostasis. Pathology international, 69(5), 249-259.

Qi, R., Dong, F., Liu, Q., Murakumo, Y., & Liu, J. (2018). CD109 and squamous cell carcinoma. Journal of translational medicine, 16(1), 1-8.

Chuang, C. H., Greenside, P. G., Rogers, Z. N., Brady, J. J., Yang, D., Ma, R. K., ... & Winslow, M. M. (2017). Molecular definition of a metastatic lung cancer state reveals a targetable CD109–Janus kinase–Stat axis. Nature medicine, 23(3), 291-300.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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