Human Recombinant CD180 protein, Biotin Conjugated, His Tag (V2LY-0526-LY6605)

Name: Human Recombinant CD180 protein, Biotin Conjugated, His Tag
SKU: V2LY-0526-LY6605
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD180 protein, Biotin Conjugated, His Tag consist of Amino Acid: 1-626 and predicts a molecular mass of 68.9 kDa.

Molecule Mass

68.9 kDa

Conjugates

Biotin

Sequence

Amino Acid: 1-626

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD180 Molecule

Function

May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity).

Biological Process

B cell proliferation involved in immune response Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Positive regulation of lipopolysaccharide-mediated signaling pathway Source: MGI

Cellular Location

Cell membrane

Topology

Extracellular: 24-626
Helical: 627-650
Cytoplasmic: 651-661

Hayden, Z., Erdő-Bonyár, S., Bóné, B., Balázs, N., Bodó, K., Illes, Z., ... & Simon, D. (2021). Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders. Journal of Immunology Research, 2021.

Miguet, L., Mayeur-Rousse, C., Eischen, A., Galoisy, A. C., Rolland, D., Gervais, C., ... & Mauvieux, L. (2021). The Contribution of CD148, CD180 and CD200 Combination in the Diagnosis of Chronic B-Cell Lymphoproliferative Disorders. Blood, 138(Supplement 1), 3520-3520.

Edwards, K., Volpi, E., Lydyard, P., Zaitseva, K., Sayed, U., Krysov, S., ... & Porakishvili, N. (2021). Expression patterns of CD180 in the lymph nodes of patients with chronic lymphocytic leukaemia. British Journal of Haematology, 195(2), e131-e134.

Dong, G., Yao, X., Yan, F., Zhang, H., Zhu, Y., Yang, Y., ... & Xiong, H. (2019). Ligation of CD180 contributes to endotoxic shock by regulating the accumulation and immunosuppressive activity of myeloid-derived suppressor cells through STAT3. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(3), 535-546.

Roe, K., Shu, G. L., Draves, K. E., Giordano, D., Pepper, M., & Clark, E. A. (2019). Targeting antigens to CD180 but not CD40 programs immature and mature B cell subsets to become efficient APCs. The Journal of Immunology, 203(7), 1715-1729.

Kikuchi, J., Kuroda, Y., Koyama, D., Osada, N., Izumi, T., Yasui, H., ... & Furukawa, Y. (2018). Myeloma cells are activated in bone marrow microenvironment by the CD180/MD-1 complex, which senses lipopolysaccharide. Cancer research, 78(7), 1766-1778.

Carreras-González, A., Navasa, N., Martín-Ruiz, I., Lavín, J. L., Azkargorta, M., Atondo, E., ... & Anguita, J. (2018). A multi-omic analysis reveals the regulatory role of CD180 during the response of macrophages to Borrelia burgdorferi. Emerging microbes & infections, 7(1), 1-13.

Yang, Y., Wang, C., Cheng, P., Zhang, X., Li, X., Hu, Y., ... & Xiong, H. (2018). CD180 Ligation inhibits TLR7-and TLR9-mediated activation of macrophages and dendritic cells through the Lyn-SHP-1/2 axis in murine lupus. Frontiers in immunology, 9, 2643.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD180 protein, His Tag (CAT#: V2LY-0526-LY6603)

Human Recombinant CD180 protein, His Tag-1 (CAT#: V2LY-0526-LY6604)

Mouse Recombinant CD180 protein, hFc Tag (CAT#: V2LY-0526-LY8845)

Mouse Recombinant CD180 protein, His Tag (CAT#: V2LY-0526-LY8846)

Mouse Recombinant CD180 protein, His Tag-1 (CAT#: V2LY-0526-LY8847)

Rhesus Recombinant CD180 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY9787)

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Mouse Recombinant CD180 protein, His Tag (CAT#: V2LY-0526-LY8846)

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Human Recombinant CD180 protein, His Tag (CAT#: V2LY-0526-LY6603)

Rabbit Anti-CD180 Recombinant Antibody (CBCNC-011) (CAT#: CBMAB-R0463-CN)

Rhesus Recombinant CD180 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY9787)

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Mouse Anti-CD180 Recombinant Antibody (MHR73) (CAT#: CBMAB-R0466-CN)

Mouse Recombinant CD180 protein, hFc Tag (CAT#: V2LY-0526-LY8845)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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