Human Recombinant CD70 protein, rFc Tag (V2LY-0526-LY2937)

Name: Human Recombinant CD70 protein, rFc Tag
SKU: V2LY-0526-LY2937
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

rFc Tag

Protein Construction

This product is Human Recombinant CD70 protein, rFc Tag consist of Amino Acid: 39-193 and predicts a molecular mass of 44.6 kDa.

Molecule Mass

44.6 kDa

Verified

HPLC

Sequence

Amino Acid: 39-193

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥85% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD70 Molecule

Alternative Names

CD70 Molecule; Tumor Necrosis Factor Ligand Superfamily Member 7; CD70 Antigen; CD27 Ligand; CD27LG; TNFSF7; CD27-L;

Function

Cytokine which is the ligand for CD27. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Biological Process

B cell mediated immunity Source: UniProtKB
B cell proliferation Source: UniProtKB
Cell-cell signaling Source: ProtInc
Extrinsic apoptotic signaling pathway Source: BHF-UCL
Positive regulation of T cell proliferation Source: GO_Central
Signal transduction Source: ProtInc
T cell mediated immunity Source: UniProtKB
Tumor necrosis factor-mediated signaling pathway Source: Reactome

Cellular Location

Membrane

Involvement in disease

Lymphoproliferative syndrome 3 (LPFS3): An autosomal recessive, early-onset immunologic disorder characterized by increased susceptibility to Epstein-Barr virus infection in B cells, abnormal B-cell proliferation and increased susceptibility to B-cell malignancies, including Hodgkin lymphoma. Patients usually have lymphadenopathy and hypogammaglobulinemia, and may suffer from recurrent infections.

Topology

Cytoplasmic: 1-17
Helical: 18-38
Extracellular: 39-193

Liu, W., Maben, Z., Wang, C., Lindquist, K. C., Li, M., Rayannavar, V., ... & Chaparro-Riggers, J. (2021). Structural delineation and phase-dependent activation of the costimulatory CD27: CD70 complex. Journal of Biological Chemistry, 297(4).

Abolhassani, H. (2021). Specific Immune Response and Cytokine Production in CD70 Deficiency. Frontiers in Pediatrics, 9, 254.

Zhang, X., Wang, Y., Zhang, D., Li, H., Zhou, Z., & Yang, R. (2020). CD70‐silenced dendritic cells induce immune tolerance in immune thrombocytopenia patients. British Journal of Haematology, 191(3), 466-475.

Tran-Nguyen, T. K., Xue, J., Feghali-Bostwick, C., Sciurba, F. C., Kass, D. J., & Duncan, S. R. (2020). CD70 activation decreases pulmonary fibroblast production of extracellular matrix proteins. American journal of respiratory cell and molecular biology, 63(2), 255-265.

Riether, C., Pabst, T., Höpner, S., Bacher, U., Hinterbrandner, M., Banz, Y., ... & Ochsenbein, A. F. (2020). Targeting CD70 with cusatuzumab eliminates acute myeloid leukemia stem cells in patients treated with hypomethylating agents. Nature medicine, 26(9), 1459-1467.

Oba, T., Hoki, T., Yamauchi, T., Keler, T., Marsh, H. C., Cao, X., & Ito, F. (2020). A critical role of CD40 and CD70 signaling in conventional type 1 dendritic cells in expansion and antitumor efficacy of adoptively transferred tumor-specific T cells. The Journal of Immunology, 205(7), 1867-1877.

Hornero, R. A., Georgiadis, C., Hua, P., Trzupek, D., He, L. Z., Qasim, W., ... & Hester, J. (2020). CD70 expression determines the therapeutic efficacy of expanded human regulatory T cells. Communications biology, 3(1), 1-17.

Bayat, P., Taghdisi, S. M., Rafatpanah, H., Abnous, K., & Ramezani, M. (2019). In vitro selection of CD70 binding aptamer and its application in a biosensor design for sensitive detection of SKOV-3 ovarian cells. Talanta, 194, 399-405.

Dhaeze, T., Tremblay, L., Lachance, C., Peelen, E., Zandee, S., Grasmuck, C., ... & Prat, A. (2019). CD70 defines a subset of proinflammatory and CNS-pathogenic TH 1/TH 17 lymphocytes and is overexpressed in multiple sclerosis. Cellular & molecular immunology, 16(7), 652-665.

O’Neill, R. E., Du, W., Mohammadpour, H., Alqassim, E., Qiu, J., Chen, G., ... & Cao, X. (2017). T Cell–Derived CD70 Delivers an Immune Checkpoint Function in Inflammatory T Cell Responses. The Journal of Immunology, 199(10), 3700-3710.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CD70 Matched Antibody Pair (234) (CAT#: APMAB-234LY)

Cynomolgus Recombinant CD70 protein, ECD, His Tag (CAT#: V2LY-0526-LY1449)

Human Recombinant CD70 protein, ECD, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2929)

Human Recombinant CD70 protein, Trimer, ECD, APC Conjugated, His Tag (CAT#: V2LY-0526-LY2930)

Human Recombinant CD70 protein, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2931)

Human Recombinant CD70 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2932)

Human Recombinant CD70 protein, hFc Tag (CAT#: V2LY-0526-LY2933)

Human Recombinant CD70 protein, hFc Tag-1 (CAT#: V2LY-0526-LY2934)

Human Recombinant CD70 protein, hFc Tag-2 (CAT#: V2LY-0526-LY2935)

Human Recombinant CD70 protein, Fc Tag (CAT#: V2LY-0526-LY2936)

Antibody Pairs

CD70 Matched Antibody Pair (234) (CAT#: APMAB-234LY)

Related Products

Human Recombinant CD70 protein, hFc Tag-1 (CAT#: V2LY-0526-LY2934)

Human Recombinant CD70 protein, hFc Tag-2 (CAT#: V2LY-0526-LY2935)

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Human Anti-CD70 Recombinant Antibody (
5A4) (CAT#: V2LY-0725-LY639)

Human Recombinant CD70 protein, ECD, AF488 Conjugated, His Tag (CAT#: V2LY-0526-LY2938)

Mouse Recombinant CD70 protein, Trimer , His Tag (CAT#: V2LY-0526-LY8064)

Rat Recombinant CD70 protein, His Tag (CAT#: V2LY-0526-LY9267)

Mouse Recombinant CD70 protein, ECD, His Tag (CAT#: V2LY-0526-LY8063)

Mouse Recombinant CD70 protein, Fc Tag-1 (CAT#: V2LY-0526-LY8066)

Cynomolgus Recombinant CD70 protein, ECD, His Tag (CAT#: V2LY-0526-LY1449)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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