Human Recombinant CD80 protein, Biotin Conjugated, hFc & AVI Tag (V2LY-0526-LY2242)

Name: Human Recombinant CD80 protein, Biotin Conjugated, hFc & AVI Tag
SKU: V2LY-0526-LY2242
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc & AVI Tag

Protein Construction

This product is Human Recombinant CD80 protein, Biotin Conjugated, hFc & AVI Tag consist of Amino Acid: 1-242 and predicts a molecular mass of 52.4 kDa.

Molecule Mass

52.4 kDa

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-242

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD80 Molecule

Alternative Names

CD80 Molecule; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; CD28LG1; CD28LG; LAB7; BB1;

Function

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
(Microbial infection) Acts as a receptor for adenovirus subgroup B.

Biological Process

Cell surface receptor signaling pathway Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Immune response Source: GO_Central
Intracellular signal transduction Source: UniProtKB
Negative regulation of T cell proliferation Source: GO_Central
Positive regulation of granulocyte macrophage colony-stimulating factor production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of signal transduction Source: UniProtKB
Positive regulation of T cell proliferation Source: GO_Central
Positive regulation of T-helper 1 cell differentiation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
T cell costimulation Source: GO_Central

Cellular Location

Membrane

Topology

Extracellular: 35-242
Helical: 243-263
Cytoplasmic: 264-288

Tekguc, M., Wing, J. B., Osaki, M., Long, J., & Sakaguchi, S. (2021). Treg-expressed CTLA-4 depletes CD80/CD86 by trogocytosis, releasing free PD-L1 on antigen-presenting cells. Proceedings of the National Academy of Sciences, 118(30).

Zhao, Y., Lee, C. K., Lin, C. H., Gassen, R. B., Xu, X., Huang, Z., ... & Hui, E. (2019). PD-L1: CD80 cis-heterodimer triggers the co-stimulatory receptor CD28 while repressing the inhibitory PD-1 and CTLA-4 pathways. Immunity, 51(6), 1059-1073.

Haabeth, O. A. W., Blake, T. R., McKinlay, C. J., Tveita, A. A., Sallets, A., Waymouth, R. M., ... & Levy, R. (2019). Local delivery of Ox40l, Cd80, and Cd86 mRNA kindles global anticancer immunity. Cancer research, 79(7), 1624-1634.

Jiménez-Uribe, A. P., Valencia-Martínez, H., Carballo-Uicab, G., Vallejo-Castillo, L., Medina-Rivero, E., Chacón-Salinas, R., ... & Pérez-Tapia, S. M. (2019). CD80 expression correlates with IL-6 production in THP-1-like macrophages costimulated with LPS and dialyzable leukocyte extract (Transferon®). Journal of immunology research, 2019.

Kleinpeter, P., Remy-Ziller, C., Winter, E., Gantzer, M., Nourtier, V., Kempf, J., ... & Marchand, J. B. (2019). By binding CD80 and CD86, the vaccinia virus M2 protein blocks their interactions with both CD28 and CTLA4 and potentiates CD80 binding to PD-L1. Journal of virology, 93(11), e00207-19.

Li, W., Kim, T. I., Kim, J. H., & Chung, H. S. (2019). Immune checkpoint PD-1/PD-L1 CTLA-4/CD80 are blocked by Rhus verniciflua Stokes and its active compounds. Molecules, 24(22), 4062.

Huang, Y., Wang, Z., Zheng, Q., Tang, J., Cai, J., Lu, Y., & Jian, J. (2018). Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology, 72, 95-103.

Siokis, A., Robert, P. A., Demetriou, P., Dustin, M. L., & Meyer-Hermann, M. (2018). F-actin-driven CD28-CD80 localization in the immune synapse. Cell reports, 24(5), 1151-1162.

Bengsch, F., Knoblock, D. M., Liu, A., McAllister, F., & Beatty, G. L. (2017). CTLA-4/CD80 pathway regulates T cell infiltration into pancreatic cancer. Cancer Immunology, Immunotherapy, 66(12), 1609-1617.

Mo, Z. Q., Wang, J. L., Yang, M., Ni, L. Y., Wang, H. Q., Lao, G. F., ... & Dan, X. M. (2017). Characterization and expression analysis of grouper (Epinephelus coioides) co-stimulatory molecules CD83 and CD80/86 post Cryptocaryon irritans infection. Fish & shellfish immunology, 67, 467-474.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus, Rhesus Recombinant CD80 protein, hFc Tag (CAT#: V2LY-0526-LY1677)

Cynomolgus, Rhesus Recombinant CD80 protein, His Tag (CAT#: V2LY-0526-LY1678)

Human Recombinant CD80 protein (CAT#: V2LY-0526-LY2238)

Human Recombinant CD80 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2239)

Human Recombinant CD80 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2240)

Human Recombinant CD80 protein, ECD, His Tag (CAT#: V2LY-0526-LY2241)

Mouse Recombinant CD80 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY7836)

Mouse Recombinant CD80 protein, hFc Tag (CAT#: V2LY-0526-LY7837)

Mouse Recombinant CD80 protein, His Tag (CAT#: V2LY-0526-LY7838)

Rat Recombinant CD80 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY9167)

Antibody Pairs

CD80 Matched Antibody Pair (236) (CAT#: APMAB-236LY)

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Human Recombinant CD80 protein (CAT#: V2LY-0526-LY2238)

Human Recombinant CD80 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2239)

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Mouse Anti-CD80 Recombinant Antibody (CBT2947) (CAT#: V2LY-0625-LY2327)

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Mouse Recombinant CD80 protein, His Tag (CAT#: V2LY-0526-LY7838)

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Rat Recombinant CD80 protein, His Tag (CAT#: V2LY-0526-LY9168)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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