Human Recombinant CD97 protein, His Tag (V2LY-0526-LY2982)

Name: Human Recombinant CD97 protein, His Tag
SKU: V2LY-0526-LY2982
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD97 protein, His Tag consist of Amino Acid: 1-398 and predicts a molecular mass of 42.6 kDa.

Molecule Mass

42.6 kDa

Sequence

Amino Acid: 1-398

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Adhesion G Protein-Coupled Receptor E5

Function

Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cell adhesion
Cell-cell signaling
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Immune response
Inflammatory response
Neutrophil degranulation

Cellular Location

Cell membrane
Adhesion G protein-coupled receptor E5 subunit alpha: Extracellular space

Topology

Extracellular: 21-552 aa
Helical: 553-572 aa
Cytoplasmic: 573-581 aa
Helical: 582-601 aa
Extracellular: 602-620 aa
Helical: 621-642 aa
Cytoplasmic: 643-653 aa
Helical: 654-674 aa
Extracellular: 675-691 aa
Helical: 692-712 aa
Cytoplasmic: 713-739 aa
Helical: 740-760 aa
Extracellular: 761-766 aa
Helical: 767-789 aa
Cytoplasmic: 790-835 aa

PTM

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD97 protein, hFc Tag (CAT#: V2LY-0526-LY2981)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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