Human Recombinant CDCP1 protein, hFc Tag (V2LY-0526-LY3004)

Name: Human Recombinant CDCP1 protein, hFc Tag
SKU: V2LY-0526-LY3004
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CDCP1 protein, hFc Tag consist of Amino Acid: 1-667 and predicts a molecular mass of 98.56 kDa.

Molecule Mass

98.56 kDa

Verified

HPLC

Sequence

Amino Acid: 1-667

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CUB Domain Containing Protein 1

Alternative Names

CUB Domain Containing Protein 1; Subtractive Immunization M Plus HEp3-Associated 135 KDa Protein; Transmembrane And Associated With Src Kinases; Membrane Glycoprotein Gp140; SIMA135; TRASK; CUB Domain-Containing Protein 1; CD318 Antigen; CD318;

Function

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.

Biological Process

Isoform 1: Cell membrane. Shedding may also lead to a soluble peptide.
Isoform 3: Secreted

Topology

Extracellular: 30-667
Helical: 668-688
Cytoplasmic: 689-836

PTM

Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.

Wang, M., Liu, D., Murthy, V., Nguyen, T. T., Lim, D. Y., Huang, R., ... & Pereira, N. (2021, May). ROLE AND FUNCTIONAL CHARACTERIZATION OF CUB-DOMAIN CONTAINING PROTEIN 1 (CDCP1) IN DILATED CARDIOMYOPATHY. In Journal of the American College of Cardiology (Vol. 77, No. 18, pp. 537-537). STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA: ELSEVIER SCIENCE INC.

Khan, T., Kryza, T., Lyons, N. J., He, Y., & Hooper, J. D. (2021). The CDCP1 Signaling Hub: A Target for Cancer Detection and Therapeutic Intervention. Cancer Research, 81(9), 2259-2269.

Mamoor, S. (2021). Over-expression of CUB domain containing protein 1 in human endometrial cancer.

Moroz, A., Wang, Y. H., Sharib, J. M., Wei, J., Zhao, N., Huang, Y., ... & Evans, M. J. (2020). Theranostic targeting of CUB domain containing protein 1 (CDCP1) in pancreatic cancer. Clinical Cancer Research, 26(14), 3608-3615.

Huang, L., Chen, Y., Lai, S., Guan, H., Hu, X., Liu, J., ... & Zhou, J. (2020). CUB Domain-Containing Protein-1 Promotes Proliferation, Migration and Invasion in Cervical Cancer Cells. Cancer management and research, 12, 3759.

Nelson, L. J., Wright, H. J., Dinh, N. B., Nguyen, K. D., Razorenova, O. V., & Heinemann, F. S. (2020). Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers. The American journal of pathology, 190(2), 484-502.

Predes, D., Cruz, J. V. R., Abreu, J. G., & Mendes, F. A. (2019). CUB domain-containing protein 1 (CDCP1) binds transforming growth factor beta family members and increase TGF-β1 signaling pathway. Experimental cell research, 383(1), 111499.

Kajiwara, K., Sugihara, A., Aoki, K., Okuzaki, D., Matsumoto, K., & Okada, M. (2019). CUB domain-containing protein 1 controls HGF responses by integrating Src and Met–STAT3 signaling. bioRxiv, 789339.

Kuhara, K., Tokuda, K., Kitagawa, T., Baron, B., Tokunaga, M., Harada, K., ... & Kuramitsu, Y. (2018). CUB domain-containing protein 1 (CDCP1) is down-regulated by Active Hexose-Correlated Compound in human pancreatic cancer cells. Anticancer research, 38(11), 6107-6111.

Noskovičová, N., Heinzelmann, K., Burgstaller, G., Behr, J., & Eickelberg, O. (2018). Cub domain-containing protein 1 negatively regulates TGF-β signaling and myofibroblast differentiation. American Journal of Physiology-Lung Cellular and Molecular Physiology, 314(5), L695-L707.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CDCP1 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY3005)

Mouse Recombinant CDCP1 protein, hFc Tag (CAT#: V2LY-0526-LY8090)

Mouse Recombinant CDCP1 protein, His Tag (CAT#: V2LY-0526-LY8091)

Rat Recombinant CDCP1 protein, ECD, His Tag (CAT#: V2LY-0526-LY9279)

Rhesus Recombinant CDCP1 protein, His Tag (CAT#: V2LY-0526-LY9690)

Human Recombinant CDCP1 (Secreted Form) protein, His Tag (CAT#: V2LY-0526-LY3006)

Human Recombinant CDCP1 (Secreted Form) protein, Fc Tag (CAT#: V2LY-0526-LY3007)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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