Human Recombinant CDH5 protein, His & hFc Tag (V2LY-0526-LY7593)

Name: Human Recombinant CDH5 protein, His & hFc Tag
SKU: V2LY-0526-LY7593
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His & hFc Tag

Protein Construction

This product is Human Recombinant CDH5 protein, His & hFc Tag consist of Amino Acid: 1-593 and predicts a molecular mass of 92 kDa.

Molecule Mass

92 kDa

Sequence

Amino Acid: 1-593

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cadherin 5

Function

Cadherins are calcium-dependent cell adhesion proteins (By similarity).
They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602).
This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity).
It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224).
Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity).
These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed:20332120).
Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed:20332120).

Biological Process

Adherens junction organization Source: ARUK-UCL
Bicellular tight junction assembly Source: ARUK-UCL
Blood vessel endothelial cell migration Source: ARUK-UCL
Blood vessel maturation Source: Ensembl
Calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: GO_Central
Cell-cell adhesion Source: ARUK-UCL
Cell-cell adhesion mediated by cadherin Source: ARUK-UCL
Cell-cell adhesion via plasma-membrane adhesion molecules Source: GO_Central
Cell-cell junction assembly Source: UniProtKB
Cell morphogenesis Source: GO_Central
Cellular calcium ion homeostasis Source: ARUK-UCL
Homophilic cell adhesion via plasma membrane adhesion molecules Source: ProtInc
Maintenance of blood-brain barrier Source: ARUK-UCL
Multicellular organism development Source: GO_Central
Negative regulation of cell population proliferation Source: Ensembl
Negative regulation of endothelial cell apoptotic process Source: ARUK-UCL
Negative regulation of inflammatory response Source: BHF-UCL
Negative regulation of microtubule polymerization Source: ARUK-UCL
Positive regulation of angiogenesis Source: BHF-UCL
Positive regulation of BMP signaling pathway Source: ARUK-UCL
Positive regulation of cell migration Source: ARUK-UCL
Positive regulation of establishment of endothelial barrier Source: UniProtKB
Positive regulation of gene expression Source: ARUK-UCL
Positive regulation of protein-containing complex assembly Source: ARUK-UCL
Positive regulation of protein dephosphorylation Source: ARUK-UCL
Protein localization to bicellular tight junction Source: ARUK-UCL
Regulation of establishment of cell polarity Source: UniProtKB
Regulation of protein phosphorylation Source: ARUK-UCL
Regulation of vascular permeability Source: ARUK-UCL
Transforming growth factor beta receptor signaling pathway Source: UniProtKB
Vasculature development Source: GO_Central

Cellular Location

Cell membrane; Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.

Topology

Extracellular: 48-599
Helical: 600-620
Cytoplasmic: 621-784

PTM

Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity).
O-glycosylated.

Magouliotis, D. E., Fergadi, M. P., Christodoulidis, G., Svokos, A. A., Svokos, K. A., Bareka, M., & Athanasiou, T. (2021). In-depth bioinformatic study of the cadherin 5 interactome in patients with thoracic aortic aneurysm unveils 8 novel biomarkers. European Journal of Cardio-Thoracic Surgery.

Michelini, S., Ricci, M., Amato, B., Gentileschi, S., Veselenyiova, D., Kenanoglu, S., ... & Bertelli, M. (2021). CDH5, a Possible New Candidate Gene for Genetic Testing of Lymphedema. Lymphatic Research and Biology.

Mamoor, S. (2020). Cadherin 5 is differentially expressed in non-small cell lung cancer and associates with patient survival.

Casal, J. I., & Bartolomé, R. A. (2019). Beyond N-cadherin, relevance of cadherins 5, 6 and 17 in cancer progression and metastasis. International journal of molecular sciences, 20(13), 3373.

Higuchi, K., Inokuchi, M., Takagi, Y., Ishikawa, T., Otsuki, S., Uetake, H., ... & Kawano, T. (2017). Cadherin 5 expression correlates with poor survival in human gastric cancer. Journal of clinical pathology, 70(3), 217-221.

Inokuchi, M., Higuchi, K., Takagi, Y., Tanioka, T., Nakagawa, M., Gokita, K., ... & Kojima, K. (2017). Cadherin 5 is a significant risk factor for hematogenous recurrence and a prognostic factor in locally advanced gastric cancer. Anticancer research, 37(12), 6807-6813.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CDH5 protein, His Tag (CAT#: V2LY-0526-LY9055)

Rat Recombinant CDH5 protein, His Tag (CAT#: V2LY-0526-LY9633)

Mouse Cdh5 ELISA Kit (CAT#: V2LY-0626-LY2102)

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Rat Anti-CDH5 Recombinant Antibody (CBYY-0996) (CAT#: CBMAB-0999-YY)

Isotype control

For research use only. Not intended for any clinical use.

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