Human Recombinant CDO protein, His Tag (V2LY-0526-LY3076)

Name: Human Recombinant CDO protein, His Tag
SKU: V2LY-0526-LY3076
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CDO protein, His Tag consist of Amino Acid: 1-963 and predicts a molecular mass of 103 kDa.

Molecule Mass

103 kDa

Sequence

Amino Acid: 1-963

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>65% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile HEPES, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cysteine Dioxygenase Type 1

Function

Initiates several important metabolic pathways related to pyruvate and several sulfurate compounds including sulfate, hypotaurine and taurine. Critical regulator of cellular cysteine concentrations. Has an important role in maintaining the hepatic concentation of intracellular free cysteine within a proper narrow range.

Biological Process

Cysteine metabolic process Source: UniProtKB
Inflammatory response Source: UniProtKB
Lactation Source: Ensembl
L-cysteine catabolic process Source: GO_Central
Response to amino acid Source: Ensembl
Response to cAMP Source: Ensembl
Response to ethanol Source: Ensembl
Response to glucagon Source: Ensembl
Response to glucocorticoid Source: Ensembl
Sulfur amino acid biosynthetic process Source: UniProtKB
Taurine biosynthetic process Source: UniProtKB

Cellular Location

Cytosol

PTM

The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe2+ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.

Dawson, P. A., Weerasekera, S. J., Atcheson, R. J., Twomey, S. A., & Simmons, D. G. (2020). Molecular analysis of the human placental cysteine dioxygenase type 1 gene. Molecular genetics and metabolism reports, 22, 100568.

Fujiyama, Y., Kumamoto, Y., Nishizawa, N., Nakamoto, S., Harada, H., Yokota, K., ... & Yamashita, K. (2020). Promoter DNA hypermethylation of the cysteine dioxygenase 1 (CDO1) gene in intraductal papillary mucinous neoplasm (IPMN). Annals of surgical oncology, 27(10), 4007-4016.

Kang, Y. P., Torrente, L., Falzone, A., Elkins, C. M., Liu, M., Asara, J. M., ... & DeNicola, G. M. (2019). Cysteine dioxygenase 1 is a metabolic liability for non-small cell lung cancer. Elife, 8, e45572.

Harada, H., Hosoda, K., Moriya, H., Mieno, H., Ema, A., Ushiku, H., ... & Yamashita, K. (2019). Cancer-specific promoter DNA methylation of Cysteine dioxygenase type 1 (CDO1) gene as an important prognostic biomarker of gastric cancer. Plos one, 14(4), e0214872.

Nishizawa, N., Harada, H., Kumamoto, Y., Kaizu, T., Katoh, H., Tajima, H., ... & Yamashita, K. (2019). Diagnostic potential of hypermethylation of the cysteine dioxygenase 1 gene (CDO 1) promoter DNA in pancreatic cancer. Cancer science, 110(9), 2846-2855.

Kojima, K., Nakamura, T., Ohbu, M., Katoh, H., Ooizumi, Y., Igarashi, K., ... & Yamashita, K. (2018). Cysteine dioxygenase type 1 (CDO1) gene promoter methylation during the adenoma-carcinoma sequence in colorectal cancer. PLoS One, 13(5), e0194785.

Kojima, K., Yamashita, K., Ushiku, H., Katoh, H., Ishii, S., Tanaka, T., ... & Watanabe, M. (2017). The clinical significance of cysteine dioxygenase type 1 methylation in Barrett esophagus adenocarcinoma. Diseases of the Esophagus, 30(3).

Hao, S., Yu, J., He, W., Huang, Q., Zhao, Y., Liang, B., ... & Shi, M. (2017). Cysteine dioxygenase 1 mediates erastin-induced ferroptosis in human gastric cancer cells. Neoplasia, 19(12), 1022-1032.

Igarashi, K., Yamashita, K., Katoh, H., Kojima, K., Ooizumi, Y., Nishizawa, N., ... & Watanabe, M. (2017). Prognostic significance of promoter DNA hypermethylation of the cysteine dioxygenase 1 (CDO1) gene in primary gallbladder cancer and gallbladder disease. PLoS One, 12(11), e0188178.

Choi, J. I., Cho, E. H., Kim, S. B., Kim, R., Kwon, J., Park, M., ... & Lee, K. H. (2017). Promoter methylation of cysteine dioxygenase type 1: gene silencing and tumorigenesis in hepatocellular carcinoma. Annals of Hepato-biliary-pancreatic Surgery, 21(4), 181-187.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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