Human Recombinant CHL1 protein, His Tag (V2LY-0526-LY3123)

Name: Human Recombinant CHL1 protein, His Tag
SKU: V2LY-0526-LY3123
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CHL1 protein, His Tag consist of Amino Acid: 1-1080 and predicts a molecular mass of 120 kDa.

Molecule Mass

120 kDa

Verified

HPLC

Sequence

Amino Acid: 1-1080

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

cell adhesion molecule with homology to L1CAM (close homolog of L1)

Function

Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane (By similarity).

Biological Process

Axon guidance Source: InterPro
Axon regeneration Source: Ensembl
Cell adhesion Source: ProtInc
Signal transduction Source: ProtInc

Cellular Location

Cell membrane. Soluble forms produced by cleavage/shedding also exist.
Processed neural cell adhesion molecule L1-like protein: Extracellular matrix

Topology

Extracellular: 25-1082
Helical: 1083-1103
Cytoplasmic: 1104-1208

PTM

Cleavage by metalloprotease ADAM8 in the extracellular part generates 2 soluble forms (125 kDa and 165 kDa) in vitro and is inhibited by metalloprotease inhibitors (By similarity). Cleaved by BACE1 (By similarity).
N-glycosylated. Contains N-linked oligosaccharides with a sulfated carbohydrate structure type HNK-1 (SO4-3-GlcUABeta1,3GalBeta1,4GlcNAc) (By similarity).
O-glycosylated.

Chen, Y., Sun, T., Niu, Y., Wang, D., Liu, K., Wang, T., ... & Liu, J. (2021). Cell adhesion molecule L1 like plays a role in the pathogenesis of idiopathic hypogonadotropic hypogonadism. Journal of Endocrinological Investigation, 1-13.

Loers, G., Theis, T., Baixia Hao, H., Kleene, R., Arsha, S., Samuel, N., ... & Schachner, M. (2021). Interplay in neural functions of cell adhesion molecule close homolog of L1 (CHL1) and Programmed Cell Death 6 (PDCD6). FASEB BioAdvances.

Yang, C. R., Ning, L., Zhou, F. H., Sun, Q., Meng, H. P., Han, Z., ... & Zhou, X. F. (2020). Downregulation of adhesion molecule CHL1 in B cells but not T cells of patients with major depression and in the brain of mice with chronic stress. Neurotoxicity research, 38(4), 914-928.

Lin, W. W., Ou, G. Y., Lin, J. Z., Yi, S. J., Yao, W. C., Pan, H. C., & Zhao, W. J. (2020). Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma. Oncology letters, 20(1), 326-336.

Karstens, K. F., Bellon, E., Polonski, A., Wolters-Eisfeld, G., Melling, N., Reeh, M., ... & Tachezy, M. (2020). Expression and serum levels of the neural cell adhesion molecule L1-like protein (CHL1) in gastrointestinal stroma tumors (GIST) and its prognostic power. Oncotarget, 11(13), 1131.

Tang, H., Jiang, L., Zhu, C., Liu, R., Wu, Y., Yan, Q., ... & Guan, X. Y. (2019). Loss of cell adhesion molecule L1 like promotes tumor growth and metastasis in esophageal squamous cell carcinoma. Oncogene, 38(17), 3119-3133.

Zhang, C., Wu, W., Ye, X., Ma, R., Luo, J., Zhu, H., & Chang, X. (2019). Aberrant expression of CHL1 gene and long non-coding RNA CHL1-AS1, CHL1-AS2 in ovarian endometriosis. European Journal of Obstetrics & Gynecology and Reproductive Biology, 236, 177-182.

Guseva, D., Jakovcevski, I., Irintchev, A., Leshchyns’Ka, I., Sytnyk, V., Ponimaskin, E., & Schachner, M. (2018). Cell adhesion molecule close homolog of L1 (CHL1) guides the regrowth of regenerating motor axons and regulates synaptic coverage of motor neurons. Frontiers in molecular neuroscience, 11, 174.

Katic, J., Loers, G., Tosic, J., Schachner, M., & Kleene, R. (2017). The cell adhesion molecule CHL1 interacts with patched-1 to regulate apoptosis during postnatal cerebellar development. Journal of cell science, 130(15), 2606-2619.

Alsanie, W. F., Penna, V., Schachner, M., Thompson, L. H., & Parish, C. L. (2017). Homophilic binding of the neural cell adhesion molecule CHL1 regulates development of ventral midbrain dopaminergic pathways. Scientific reports, 7(1), 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CHL1 protein (CAT#: V2LY-0526-LY8107)

Mouse Recombinant CHL1 protein, hFc Tag (CAT#: V2LY-0526-LY8108)

Mouse Recombinant CHL1 protein, His Tag (CAT#: V2LY-0526-LY8109)

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Mouse Recombinant CHL1 protein, hFc Tag (CAT#: V2LY-0526-LY8108)

Mouse Recombinant CHL1 protein, His Tag (CAT#: V2LY-0526-LY8109)

Mouse Recombinant CHL1 protein (CAT#: V2LY-0526-LY8107)

Mouse Anti-CHL1 Recombinant Antibody (CBFYC-1839) (CAT#: CBMAB-C1903-FY)

Rat Anti-CHL1 Recombinant Antibody (CBYY-C2061) (CAT#: CBMAB-C3499-YY)

Mouse Anti-CHL1 Recombinant Antibody (CBXC-1720) (CAT#: CBMAB-C3631-CQ)

Mouse Anti-CHL1 Recombinant Antibody (CBYY-C2062) (CAT#: CBMAB-C3500-YY)

Rat Anti-CHL1 Recombinant Antibody (CBXC-1095) (CAT#: CBMAB-C3632-CQ)

Isotype control

For research use only. Not intended for any clinical use.

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