Human Recombinant CHST11 protein, His Tag (V2LY-0526-LY3132)

Name: Human Recombinant CHST11 protein, His Tag
SKU: V2LY-0526-LY3132
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CHST11 protein, His Tag consist of Amino Acid: 36-347 and predicts a molecular mass of 38.4 kDa.

Molecule Mass

38.4 kDa

Sequence

Amino Acid: 36-347

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

carbohydrate (chondroitin 4) sulfotransferase 11

Function

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues in desulfated dermatan sulfate. Preferentially sulfates in GlcA->GalNAc unit than in IdoA->GalNAc unit. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.

Biological Process

Carbohydrate biosynthetic process Source: InterPro
Chondrocyte development Source: Ensembl
Chondroitin sulfate biosynthetic process Source: UniProtKB
Developmental growth Source: Ensembl
Embryonic digit morphogenesis Source: Ensembl
Embryonic viscerocranium morphogenesis Source: Ensembl
In utero embryonic development Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of transforming growth factor beta receptor signaling pathway Source: Ensembl
Polysaccharide localization Source: Ensembl
Post-anal tail morphogenesis Source: Ensembl
Post-embryonic development Source: Ensembl
Proteoglycan biosynthetic process Source: GO_Central
Regulation of cell population proliferation Source: Ensembl
Respiratory gaseous exchange by respiratory system Source: Ensembl

Cellular Location

Golgi apparatus membrane

Involvement in disease

A chromosomal aberration involving CHST11 is found in B-cell chronic lymphocytic leukemias. Translocation t(12;14)(q23;q32) with IgH.
Osteochondrodysplasia, brachydactyly, and overlapping malformed digits (OCBMD):
An autosomal recessive disorder characterized by bilateral symmetric skeletal defects that primarily affect the limbs. Affected individuals have mild short stature due to shortening of the lower leg bones, as well as hand and foot malformations, predominantly brachydactyly and overlapping digits. Other skeletal defects include scoliosis, dislocated patellae and fibulae, and pectus excavatum.

Topology

Cytoplasmic: 1-16
Helical: 17-37
Lumenal: 38-352

PTM

N-glycosylated; required for activity and stability.

Mamoor, S. (2021). Differential expression of carbohydrate (chondroitin 4) sulfotransferase 11 in human epithelial ovarian cancer.

Behrens, A., Jousheghany, F., Yao-Borengasser, A., Siegel, E. R., Kieber-Emmons, T., & Monzavi-Karbassi, B. (2020). Carbohydrate (chondroitin 4) sulfotransferase-11-mediated induction of epithelial-mesenchymal transition and generation of cancer stem cells. Pharmacology, 105(5-6), 246-259.

Zhang, L., Shi, R., Ouyang, H., & Li, Y. (2020). Cloning and characterization of chst11 from Procambarus clarkii involved in the host immune response of white spot syndrome virus and Aeromonas hydrophila. Fish & shellfish immunology, 102, 82-91.

Shabbir, R. M. K., Nalbant, G., Ahmad, N., Malik, S., & Tolun, A. (2018). Homozygous CHST11 mutation in chondrodysplasia, brachydactyly, overriding digits, clino-symphalangism and synpolydactyly. Journal of medical genetics, 55(7), 489-496.

Wang, Q., Yang, C., Hao, R., Zheng, Z., Jiao, Y., Du, X., ... & Huang, R. (2017). Molecular characterization of CHST11 and its potential role in nacre formation in pearl oyster Pinctada fucata martensii. Electronic Journal of Biotechnology, 28, 113-119.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human CHST11 ELISA Kit (CAT#: V2LY-0626-LY3494)

Related Products

Mouse Anti-CHST11 Recombinant Antibody (CBFYC-1861) (CAT#: CBMAB-C1925-FY)

Human CHST11 ELISA Kit (CAT#: V2LY-0626-LY3494)

Mouse Anti-CHST11 Recombinant Antibody (CBFYC-1862) (CAT#: CBMAB-C1926-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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