Human Recombinant CNTF protein, His Tag (V2LY-0526-LY3303)

Name: Human Recombinant CNTF protein, His Tag
SKU: V2LY-0526-LY3303
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CNTF protein, His Tag consist of Amino Acid: 2-200 and predicts a molecular mass of 24.5 kDa.

Molecule Mass

24.5 kDa

Sequence

Amino Acid: 2-200

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Ciliary Neurotrophic Factor

Function

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Biological Process

Astrocyte activation Source: GO_Central
Ciliary neurotrophic factor-mediated signaling pathway Source: BHF-UCL
Cytokine-mediated signaling pathway Source: Reactome
Muscle organ morphogenesis Source: Ensembl
Negative regulation of neuron apoptotic process Source: BHF-UCL
Negative regulation of photoreceptor cell differentiation Source: Ensembl
Neuron development Source: Ensembl
Positive regulation of axon regeneration Source: GO_Central
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of gene expression Source: MGI
Positive regulation of tyrosine phosphorylation of STAT protein Source: BHF-UCL
Regulation of retinal cell programmed cell death Source: Ensembl
Signal transduction Source: ProtInc

Cellular Location

Cytoplasm

Wang, Y., Rhee, K. D., Pellegrini, M., & Yang, X. J. (2020). Impacts of ciliary neurotrophic factor on the retinal transcriptome in a mouse model of photoreceptor degeneration. Scientific reports, 10(1), 1-11.

Chew, E. Y., Clemons, T. E., Jaffe, G. J., Johnson, C. A., Farsiu, S., Lad, E. M., ... & Phase 2 CNTF Research Group. (2019). Effect of ciliary neurotrophic factor on retinal neurodegeneration in patients with macular telangiectasia type 2: a randomized clinical trial. Ophthalmology, 126(4), 540-549.

Jia, C., Brown, R. W., Malone, H. M., Burgess, K. C., Gill, W. D., Keasey, M. P., & Hagg, T. (2019). Ciliary neurotrophic factor is a key sex-specific regulator of depressive-like behavior in mice. Psychoneuroendocrinology, 100, 96-105.

Ghasemi, M., Alizadeh, E., Saei Arezoumand, K., Fallahi Motlagh, B., & Zarghami, N. (2018). Ciliary neurotrophic factor (CNTF) delivery to retina: an overview of current research advancements. Artificial cells, nanomedicine, and biotechnology, 46(8), 1694-1707.

Li, S., Sato, K., Gordon, W. C., Sendtner, M., Bazan, N. G., & Jin, M. (2018). Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments. Journal of Biological Chemistry, 293(39), 15256-15268.

Zheng, K., Zhang, Q., Sheng, Z., Li, Y., & Lu, H. H. (2018). Ciliary neurotrophic factor (CNTF) protects myocardial cells from oxygen glucose deprivation (OGD)/re-oxygenation via activation of Akt-Nrf2 signaling. Cellular Physiology and Biochemistry, 51(4), 1852-1862.

Baek, J. Y., Jeong, J. Y., Kim, K. I., Won, S. Y., Chung, Y. C., Nam, J. H., ... & Jin, B. K. (2018). Inhibition of microglia-derived oxidative stress by ciliary neurotrophic factor protects dopamine neurons in vivo from MPP+ neurotoxicity. International journal of molecular sciences, 19(11), 3543.

Ma, M., Xu, Y., Xiong, S., Zhang, J., Gu, Q., Ke, B., & Xu, X. (2018). Involvement of ciliary neurotrophic factor in early diabetic retinal neuropathy in streptozotocin-induced diabetic rats. Eye, 32(9), 1463-1471.

Shpak, A. A., Guekht, A. B., Druzhkova, T. A., Kozlova, K. I., & Gulyaeva, N. V. (2017). Ciliary neurotrophic factor in patients with primary open-angle glaucoma and age-related cataract. Molecular vision, 23, 799.

Fan, L., Xiong, Y., Fu, Z., Xu, D., Wang, L., Chen, Y., ... & Ye, Q. (2017). Polyaniline promotes peripheral nerve regeneration by enhancement of the brain‑derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling pathway. Molecular medicine reports, 16(5), 7534-7540.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CNTFR protein, His Tag (CAT#: V2LY-0526-LY3304)

Mouse Recombinant CNTF protein, His Tag (CAT#: V2LY-0526-LY8140)

Rat Recombinant CNTF protein (CAT#: V2LY-0526-LY9301)

Human CNTF ELISA Kit (CAT#: V2LY-0626-LY1478)

Mouse Cntf ELISA Kit (CAT#: V2LY-0626-LY725)

Rat Cntf ELISA Kit (CAT#: V2LY-0626-LY788)

CNTF Matched Antibody Pair (283) (CAT#: APMAB-283LY)

Antibody Pairs

CNTF Matched Antibody Pair (283) (CAT#: APMAB-283LY)

Related Products

Human Recombinant CNTFR protein, His Tag (CAT#: V2LY-0526-LY3304)

Human CNTF ELISA Kit (CAT#: V2LY-0626-LY1478)

Rat Recombinant CNTF protein (CAT#: V2LY-0526-LY9301)

Mouse Anti-CNTF Recombinant Antibody (C4008) (CAT#: V2LY-1206-LY520)

Mouse Recombinant CNTF protein, His Tag (CAT#: V2LY-0526-LY8140)

Mouse Anti-CNTF Recombinant Antibody (CBFYC-2009) (CAT#: V2LY-1206-LY513)

Rabbit Anti-CNTF Recombinant Antibody (1) (CAT#: V2LY-1206-LY510)

Mouse Anti-CNTF Recombinant Antibody (CBCNC-038) (CAT#: V2LY-1206-LY521)

Mouse Anti-CNTF Recombinant Antibody (CBFYC-2012) (CAT#: V2LY-1206-LY514)

Mouse Anti-CNTF Recombinant Antibody (CBYY-C2958) (CAT#: V2LY-1206-LY518)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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