Human Recombinant CNTN5 protein, His Tag (V2LY-0526-LY3305)

Name: Human Recombinant CNTN5 protein, His Tag
SKU: V2LY-0526-LY3305
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CNTN5 protein, His Tag consist of Amino Acid: 1-1059 and predicts a molecular mass of 116 kDa.

Molecule Mass

116 kDa

Sequence

Amino Acid: 1-1059

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Contactin 5

Function

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Probably involved in neuronal activity in the auditory system (By similarity).

Biological Process

Cell adhesion Source: UniProtKB-KW
Presynapse assembly Source: Ensembl
Sensory perception of sound Source: Ensembl

Cellular Location

Cell membrane

Schmilovich, Z., Huguet, G., He, Q., Musa-Johnson, A., Douard, E., Loum, M. A., ... & Rouleau, G. A. (2021). Copy-Number Variants in The Contactin-5 Gene Are a Potential Risk Factor for Autism Spectrum Disorder.

Dauar, M. T., Picard, C., Rosa‐Neto, P., Villeneuve, S., Poirier, J., & Prevent‐AD Research Group. (2021). CNTN5 is associated with disease risk and pathology throughout the Alzheimer’s disease continuum. Alzheimer's & Dementia, 17, e052359.

Kleijer, K. T., van Nieuwenhuize, D., Spierenburg, H. A., Gregorio-Jordan, S., Kas, M. J., & Burbach, J. P. H. (2018). Structural abnormalities in the primary somatosensory cortex and a normal behavioral profile in Contactin-5 deficient mice. Cell Adhesion & Migration, 12(1), 5-18.

Smirnov, A. V., Kontsevaya, G. V., Feofanova, N. A., Anisimova, M. V., Serova, I. A., Gerlinskaya, L. A., ... & Serov, O. L. (2018). Unexpected phenotypic effects of a transgene integration causing a knockout of the endogenous Contactin-5 gene in mice. Transgenic research, 27(1), 1-13.

Peng, Y. R., Tran, N. M., Krishnaswamy, A., Kostadinov, D., Martersteck, E. M., & Sanes, J. R. (2017). Satb1 regulates contactin 5 to pattern dendrites of a mammalian retinal ganglion cell. Neuron, 95(4), 869-883.

Oguro-Ando, A., Zuko, A., Kleijer, K. T., & Burbach, J. P. H. (2017). A current view on contactin-4,-5, and-6: Implications in neurodevelopmental disorders. Molecular and Cellular Neuroscience, 81, 72-83.

Smirnov, A. V., Feofanova, N. A., Kontsevaya, G. V., Anisimova, M. V., Kovrigin, I. I., Serova, I. A., ... & Battulin, N. R. (2017). Morphophysiological effects of insertional mutagenesis of the contactin 5 (Cntn5) gene in transgenic mice. Russian Journal of Genetics: Applied Research, 7(8), 799-805.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CNTN5 protein, His Tag (CAT#: V2LY-0526-LY8141)

Human CNTN5 ELISA Kit (CAT#: V2LY-0626-LY4440)

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Rabbit Anti-CNTN5 Recombinant Antibody (024) (CAT#: V2LY-1206-LY545)

Human CNTN5 ELISA Kit (CAT#: V2LY-0626-LY4440)

Mouse Recombinant CNTN5 protein, His Tag (CAT#: V2LY-0526-LY8141)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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