Human Recombinant CSF2, GMP grade protein (V2LY-0526-LY4362)

Name: Human Recombinant CSF2, GMP grade protein
SKU: V2LY-0526-LY4362
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Protein Construction

This product is Human Recombinant CSF2, GMP grade protein consist of Amino Acid: 1-144 and predicts a molecular mass of 14.5 kDa.

Molecule Mass

14.5 kDa

Verified

HPLC

Sequence

Amino Acid: 1-144

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

<10 EU/mg of the protein.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to thirty-six months from date of receipt at ≤ -20°C
Store it under sterile conditions at ≤-20°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Colony Stimulating Factor 2

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Biological Process

Cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: CAFA
Cellular response to lipopolysaccharide Source: Ensembl
Cytokine-mediated signaling pathway Source: Reactome
Dendritic cell differentiation Source: DFLAT
Embryonic placenta development Source: Ensembl
Epithelial fluid transport Source: Ensembl
Histamine secretion Source: Ensembl
Immune response Source: InterPro
Macrophage activation Source: Ensembl
Macrophage differentiation Source: ARUK-UCL
MAPK cascade Source: Reactome
Monocyte differentiation Source: Ensembl
Myeloid cell differentiation Source: GO_Central
Myeloid dendritic cell differentiation Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Neutrophil differentiation Source: Ensembl
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of gene expression Source: BHF-UCL
Positive regulation of interleukin-23 production Source: BHF-UCL
Positive regulation of macrophage derived foam cell differentiation Source: BHF-UCL
Positive regulation of podosome assembly Source: BHF-UCL
Positive regulation of tyrosine phosphorylation of STAT protein Source: BHF-UCL
Regulation of circadian sleep/wake cycle, sleep Source: Ensembl
Regulation of myeloid cell differentiation Source: Reactome
Response to fluid shear stress Source: Ensembl
Response to silicon dioxide Source: Ensembl

Cellular Location

Secreted

Xiao, Y., Uh, K., Negrón-Pérez, V. M., Haines, H., Lee, K., & Hansen, P. J. (2021). Regulation of gene expression in the bovine blastocyst by colony-stimulating factor 2 is disrupted by CRISPR/Cas9-mediated deletion of CSF2RA. Biology of Reproduction, 104(5), 995-1007.

Estrada-Cortés, E., Jannaman, E. A., Block, J., Amaral, T. F., & Hansen, P. J. (2021). Programming of postnatal phenotype caused by exposure of cultured embryos from Brahman cattle to colony-stimulating factor 2 and serum. Journal of Animal Science, 99(8), skab180.

Sinkey, R. G., Guzeloglu-Kayisli, O., Arlier, S., Guo, X., Semerci, N., Moore, R., ... & Lockwood, C. J. (2020). Thrombin-induced decidual colony-stimulating factor-2 promotes abruption-related preterm birth by weakening fetal membranes. The American journal of pathology, 190(2), 388-399.

Sosa, F., Block, J., Xiao, Y., & Hansen, P. J. (2020). Determinants of survival of the bovine blastocyst to cryopreservation stress: treatment with colony stimulating factor 2 during the morula-to-blastocyst transition and embryo sex. CABI agriculture and bioscience, 1(1), 1-10.

Sielska, M., Przanowski, P., Pasierbińska, M., Wojnicki, K., Poleszak, K., Wojtas, B., ... & Kaminska, B. (2020). Tumour-derived CSF2/granulocyte macrophage colony stimulating factor controls myeloid cell accumulation and progression of gliomas. British journal of cancer, 123(3), 438-448.

Sharkey, D. J., Glynn, D. J., Schjenken, J. E., Tremellen, K. P., & Robertson, S. A. (2018). Interferon-gamma inhibits seminal plasma induction of colony-stimulating factor 2 in mouse and human reproductive tract epithelial cells. Biology of reproduction, 99(3), 514-526.

Tríbulo, P., Bernal Ballesteros, B. H., Ruiz, A., Tríbulo, A., Tríbulo, R. J., Tríbulo, H. E., ... & Hansen, P. J. (2017). Consequences of exposure of embryos produced in vitro in a serum-containing medium to dickkopf-related protein 1 and colony stimulating factor 2 on blastocyst yield, pregnancy rate, and birth weight. Journal of animal science, 95(10), 4407-4412.

Siqueira, L. G., Tribulo, P., Chen, Z., Denicol, A. C., Ortega, M. S., Negrón-Pérez, V. M., ... & Hansen, P. J. (2017). Colony-stimulating factor 2 acts from days 5 to 7 of development to modify programming of the bovine conceptus at day 86 of gestation. Biology of Reproduction, 96(4), 743-757.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Csf2 ELISA Kit (CAT#: V2LY-0626-LY216)

Cattle CSF2 ELISA Kit (CAT#: V2LY-0626-LY2534)

Human CSF2 ELISA Kit (CAT#: V2LY-0626-LY372)

Dog CSF2 ELISA Kit (CAT#: V2LY-0626-LY446)

Sheep CSF2 ELISA Kit (CAT#: V2LY-0626-LY545)

Rat Csf2 ELISA Kit (CAT#: V2LY-0626-LY869)

Cattle CSF2 ELISA Kit (2) (CAT#: V2LY-0626-LY870)

Pig CSF2 ELISA Kit (CAT#: V2LY-0626-LY967)

Human Recombinant CSF2 protein, hFc Tag (CAT#: V2LY-0526-LY4359)

Human Recombinant CSF2 protein, His Tag (CAT#: V2LY-0526-LY4360)

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Pig CSF2 ELISA Kit (CAT#: V2LY-0626-LY967)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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