Human Recombinant CSTA protein, His Tag (V2LY-0526-LY3473)

Name: Human Recombinant CSTA protein, His Tag
SKU: V2LY-0526-LY3473
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CSTA protein, His Tag consist of Amino Acid: 2-98 and predicts a molecular mass of 12.4 kDa.

Molecule Mass

12.4 kDa

Sequence

Amino Acid: 2-98

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CSTA

Function

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.

Biological Process

Cell-cell adhesion Source: UniProtKB
Cornification Source: Reactome
Keratinocyte differentiation Source: UniProtKB
Negative regulation of peptidase activity Source: BHF-UCL
Negative regulation of proteolysis Source: UniProtKB
Peptide cross-linking Source: UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Peeling skin syndrome 4 (PSS4):
A genodermatosis characterized by congenital exfoliative ichthyosis, sharing some features with ichthyosis bullosa of Siemens and annular epidermolytic ichthyosis. PSS4 presents shortly after birth as dry, scaly skin over most of the body with coarse peeling of non-erythematous skin on the palms and soles, which is exacerbated by excessive moisture and minor trauma. Electron microscopy analysis of skin biopsies, reveals mostly normal-appearing upper layers of the epidermis, but prominent intercellular edema of the basal and suprabasal cell layers with aggregates of tonofilaments in the basal keratinocytes.

Mary, D. J. S. J., Sikarwar, G., Kumar, A., & Limaye, A. M. (2020). Interplay of ERα binding and DNA methylation in the intron-2 determines the expression and estrogen regulation of cystatin A in breast cancer cells. Molecular and Cellular Endocrinology, 504, 110701.

Mezzapesa, A., Bastelica, D., Crescence, L., Poggi, M., Grino, M., Peiretti, F., ... & Canault, M. (2019). Increased levels of the megakaryocyte and platelet expressed cysteine proteases stefin A and cystatin A prevent thrombosis. Scientific reports, 9(1), 1-15.

Dohm, A., Su, J., McTyre, E. R., Taylor, J. M., Miller, L. D., Petty, W. J., ... & Ruiz, J. (2019). Identification of CD37, cystatin A, and IL-23A gene expression in association with brain metastasis: Analysis of a prospective trial. The International journal of biological markers, 34(1), 90-97.

Ma, Y., Chen, Y., Li, Y., Grün, K., Berndt, A., Zhou, Z., & Petersen, I. (2018). Cystatin A suppresses tumor cell growth through inhibiting epithelial to mesenchymal transition in human lung cancer. Oncotarget, 9(18), 14084.

Shiba, D., Terayama, M., Yamada, K., Hagiwara, T., Oyama, C., Tamura-Nakano, M., ... & Kawamura, Y. I. (2018). Clinicopathological significance of cystatin A expression in progression of esophageal squamous cell carcinoma. Medicine, 97(15).

Komura, T., Takabatake, H., Harada, K., Yamato, M., Miyazawa, M., Yoshida, K., ... & Sakai, Y. (2017). Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer science, 108(11), 2122-2129.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Csta ELISA Kit (CAT#: V2LY-0626-LY2252)

Human CSTA ELISA Kit (CAT#: V2LY-0626-LY4059)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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