Human Recombinant ECE1 protein, His Tag (V2LY-0526-LY3677)

Name: Human Recombinant ECE1 protein, His Tag
SKU: V2LY-0526-LY3677
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ECE1 protein, His Tag consist of Amino Acid: 90-770 and predicts a molecular mass of 80 kDa.

Molecule Mass

80 kDa

Sequence

Amino Acid: 90-770

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Endothelin Converting Enzyme 1

Research Area

Converts big endothelin-1 to endothelin-1.

Biological Process

Bradykinin catabolic process Source: BHF-UCL
Calcitonin catabolic process Source: BHF-UCL
Ear development Source: BHF-UCL
Embryonic digit morphogenesis Source: BHF-UCL
Endothelin maturation Source: BHF-UCL
G protein-coupled receptor signaling pathway Source: Reactome
Heart development Source: BHF-UCL
Hormone catabolic process Source: BHF-UCL
Peptide hormone processing Source: BHF-UCL
Pharyngeal system development Source: Ensembl
Positive regulation of receptor recycling Source: BHF-UCL
Protein processing Source: BHF-UCL
Regulation of systemic arterial blood pressure by endothelin Source: BHF-UCL
Regulation of vasoconstriction Source: BHF-UCL
Substance P catabolic process Source: BHF-UCL

Cellular Location

Cell membrane

Involvement in disease

Hirschsprung disease, cardiac defects, and autonomic dysfunction (HCAD):
A disorder characterized by skip-lesions Hirschsprung disease, craniofacial abnormalities and other dysmorphic features, cardiac defects including ductus arteriosus, small subaortic ventricular septal defect, small atrial septal defect, and autonomic dysfunction.

Topology

Cytoplasmic: 1-68
Helical: 69-89
Extracellular: 90-770

Xie, H. (2022). Investigating the Potential Roles of Human (Endothelin convert enzyme-1) ECE-1 in Congenital Heart Diseases Using a Humanized Yeast System of Apoptosis (Doctoral dissertation, University of Leicester).

Hernández-Jiménez, C., Olmos-Zúñiga, J. R., Baltazares-Lipp, M., Jasso-Victoria, R., Polo-Jerez, A., Pérez-López, M. T., ... & Silva-Martínez, M. (2022). Endothelin-Converting Enzyme 1 and Vascular Endothelial Growth Factor as Potential Biomarkers during Ex Vivo Lung Perfusion with Prolonged Hypothermic Lung-Sparing. Disease Markers, 2022.

Arfian, N., Suzuki, Y., Hartopo, A. B., Anggorowati, N., Nugrahaningsih, D. A. A., & Emoto, N. (2020). Endothelin converting enzyme-1 (ECE-1) deletion in association with endothelin-1 downregulation ameliorates kidney fibrosis in mice. Life Sciences, 258, 118223.

McCormick, A., Kristianto, J., Wang, X., McIntosh, J., Cruz, M., Hibbard, J. U., & Blank, R. D. (2020). Placental endothelin-converting enzyme-1 is decreased in preeclampsia. Pregnancy hypertension, 20, 108-110.

Tapia, J. C., & Niechi, I. (2019). Endothelin-converting enzyme-1 in cancer aggressiveness. Cancer Letters, 452, 152-157.

Cho, T. J., Kim, H. J., & Cho, J. (2019). Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis. Animal Cells and Systems, 23(3), 170-175.

McCormick, A., Kristianto, J., Wang, X., Hibbard, J., McIntosh, J., Cruz, M., & Blank, R. (2019). 428: Preeclampsia is characterized by decreased expression of endothelin converting enzyme-1 in the placenta. American Journal of Obstetrics & Gynecology, 220(1), S290.

Heyman, S. N., Abassi, Z., Rosenberger, C., Yaseen, H., Skarjinski, G., Shina, A., ... & Khamaisi, M. (2018). Cyclosporine A induces endothelin‐converting enzyme‐1: Studies in vivo and in vitro. Acta Physiologica, 223(1), e13033.

Huangfu, W. (2018). GW29-e1565 Correlation of single-nucleotide polymorphisms of the 1C3435T and ECE-1 genes with the efficacy of irbesartan in primary hypertension. Journal of the American College of Cardiology, 72(16S), C157-C158.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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