Human Recombinant ECSIT protein, His Tag (V2LY-0526-LY3682)

Name: Human Recombinant ECSIT protein, His Tag
SKU: V2LY-0526-LY3682
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ECSIT protein, His Tag consist of Amino Acid: 246-431 and predicts a molecular mass of 22.9 kDa.

Molecule Mass

22.9 kDa

Sequence

Amino Acid: 246-431

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ECSIT homolog (Drosophila)

Research Area

Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).

Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.

Biological Process

Innate immune response Source: UniProtKB-KW
Regulation of oxidoreductase activity Source: UniProtKB
Regulation of protein complex stability Source: UniProtKB

Cellular Location

Nucleus; Mitochondrion; Cytoplasm

Chaitanya, N. S., Tammineni, P., Nagaraju, G. P., & Reddy, A. B. (2022). Pleiotropic roles of evolutionarily conserved signaling intermediate in toll pathway (ECSIT) in pathophysiology. Journal of Cellular Physiology.

Qi, Z., Pi, X., Xu, Y., Zhang, Q., Wangkahart, E., Meng, F., & Wang, Z. (2022). Molecular characterization of the evolutionary conserved signaling intermediate in Toll pathways (ECSIT) of soiny mullet (Liza haematocheila). Fish & Shellfish Immunology.

Hu, Y., Liu, W., Zhang, X., & Liu, D. (2022). ECSIT inhibits cell death to increase tumor progression and metastasis via p53 in human breast cancer. Translational Cancer Research, 11(4), 699.

Xu, L., Humphries, F., Delagic, N., Wang, B., Holland, A., Edgar, K. S., ... & Moynagh, P. N. (2021). ECSIT is a critical limiting factor for cardiac function. JCI insight, 6(12).

Ge, Q., Wang, J., Li, J., & Li, J. (2021). Identification, characterization, and functional analysis of Toll and ECSIT in Exopalaemon carinicauda. Developmental & Comparative Immunology, 116, 103926.

Kim, M. J., Min, Y., Kwon, J., Son, J., Im, J. S., Shin, J., & Lee, K. Y. (2019). p62 negatively regulates TLR4 signaling via functional regulation of the TRAF6-ECSIT complex. Immune Network, 19(3).

Kim, M. J., Min, Y., Shim, J. H., Chun, E., & Lee, K. Y. (2019). CRBN is a negative regulator of bactericidal activity and autophagy activation through inhibiting the ubiquitination of ECSIT and BECN1. Frontiers in immunology, 10, 2203.

Wen, H., Ma, H., Cai, Q., Lin, S., Lei, X., He, B., ... & Liu, Q. (2018). Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma. Nature medicine, 24(2), 154-164.

Carneiro, F. R., Lepelley, A., Seeley, J. J., Hayden, M. S., & Ghosh, S. (2018). An essential role for ECSIT in mitochondrial complex I assembly and mitophagy in macrophages. Cell reports, 22(10), 2654-2666.

Wang, H., Behrens, E. M., Pignolo, R. J., & Kaplan, F. S. (2018). ECSIT links TLR and BMP signaling in FOP connective tissue progenitor cells. Bone, 109, 201-209.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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