Human Recombinant EFNA1 protein, hFc Tag (V2LY-0526-LY3925)

Name: Human Recombinant EFNA1 protein, hFc Tag
SKU: V2LY-0526-LY3925
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant EFNA1 protein, hFc Tag consist of Amino Acid: 1-182 and predicts a molecular mass of 46.08 kDa.

Molecule Mass

46.08 kDa

Sequence

Amino Acid: 1-182

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Ephrin A1

Research Area

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.

Biological Process

Angiogenesis Source: UniProtKB-KW
Aortic valve morphogenesis Source: BHF-UCL
Axon guidance Source: GO_Central
Cell-cell signaling Source: ProtInc
Cell migration Source: UniProtKB
Endocardial cushion to mesenchymal transition involved in heart valve formation Source: BHF-UCL
Ephrin receptor signaling pathway Source: UniProtKB
Mitral valve morphogenesis Source: BHF-UCL
Negative regulation of dendritic spine morphogenesis Source: UniProtKB
Negative regulation of epithelial to mesenchymal transition Source: BHF-UCL
Negative regulation of MAPK cascade Source: Ensembl
Negative regulation of proteolysis involved in cellular protein catabolic process Source: ARUK-UCL
Negative regulation of thymocyte apoptotic process Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Notochord formation Source: Ensembl
Positive regulation of amyloid-beta formation Source: ARUK-UCL
Positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process Source: ARUK-UCL
Positive regulation of MAPK cascade Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of protein phosphorylation Source: ARUK-UCL
Positive regulation of protein tyrosine kinase activity Source: ARUK-UCL
Protein stabilization Source: ARUK-UCL
Regulation of angiogenesis Source: Ensembl
Regulation of axonogenesis Source: Ensembl
Regulation of blood vessel endothelial cell migration Source: Ensembl
Regulation of cell adhesion mediated by integrin Source: UniProtKB
Regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Substrate adhesion-dependent cell spreading Source: UniProtKB

Cellular Location

Cell membrane
Ephrin-A1, secreted form: Secreted

PTM

Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form.
N-Glycosylation is required for binding to EPHA2 receptor and inducing its internalization.

Chu, L. Y., Huang, B. L., Huang, X. C., Peng, Y. H., Xie, J. J., & Xu, Y. W. (2022). EFNA1 in gastrointestinal cancer: Expression, regulation and clinical significance. World Journal of Gastrointestinal Oncology, 14(5), 973.

Shen, X., Li, M., Lei, Y., Lu, S., Wang, S., Liu, Z., ... & Zhu, G. (2022). An integrated analysis of single-cell and bulk transcriptomics reveals EFNA1 as a novel prognostic biomarker for cervical cancer. Human Cell, 35(2), 705-720.

Zhang, Y., Zhang, J., Pan, G., Guan, T., Zhang, C., Hao, A., ... & Ren, H. (2021). Effects of EFNA1 on cell phenotype and prognosis of esophageal carcinoma. World journal of surgical oncology, 19(1), 1-10.

Hao, Y. P., Wang, W. Y., Qiao, Q., & Li, G. (2021). EFNA1 is a potential key gene that correlates with immune infiltration in low-grade glioma. Medicine, 100(22).

Mamoor, S. (2021). Differential expression of EFNA1 in cancers of the breast.

Hao, Y., & Li, G. (2020). Role of EFNA1 in tumorigenesis and prospects for cancer therapy. Biomedicine & Pharmacotherapy, 130, 110567.

Asadian, F., Ghadyani, M., Antikchi, M. H., Dastgheib, S. A., Neamatzadeh, H., Sheikhpour, E., ... & Tabei, S. S. (2020). Association of Epidermal Growth Factor 61A> G, Survivin-31G> C, and EFNA1-1732G> A Polymorphisms with Susceptibility to Colorectal Cancer. Journal of Gastrointestinal Cancer, 1-6.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human EFNA1 ELISA Kit (CAT#: V2LY-0626-LY4554)

Mouse Efna1 ELISA Kit (CAT#: V2LY-0626-LY5834)

Human Recombinant EFNA1 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY3926)

Human Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY3927)

Mouse Recombinant EFNA1 protein, hFc Tag (CAT#: V2LY-0526-LY8269)

Mouse Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY8270)

Rat Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY9365)

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Human EFNA1 ELISA Kit (CAT#: V2LY-0626-LY4554)

Mouse Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY8270)

Rat Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY9365)

Human Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY3927)

Mouse Efna1 ELISA Kit (CAT#: V2LY-0626-LY5834)

Mouse Anti-EFNA1 Recombinant Antibody (1177) (CAT#: V2LY-0425-LY1499)

Rabbit Anti-EFNA1 Recombinant Antibody (453) (CAT#: V2LY-0425-LY1500)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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