Mouse Efna1 ELISA Kit (V2LY-0626-LY5834)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.023 ng/mL
Detection Range
0.05-10 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
23.8 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Ephrin A1
Research Area
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.
Biological Process
Angiogenesis Source: UniProtKB-KW
Aortic valve morphogenesis Source: BHF-UCL
Axon guidance Source: GO_Central
Cell-cell signaling Source: ProtInc
Cell migration Source: UniProtKB
Endocardial cushion to mesenchymal transition involved in heart valve formation Source: BHF-UCL
Ephrin receptor signaling pathway Source: UniProtKB
Mitral valve morphogenesis Source: BHF-UCL
Negative regulation of dendritic spine morphogenesis Source: UniProtKB
Negative regulation of epithelial to mesenchymal transition Source: BHF-UCL
Negative regulation of MAPK cascade Source: Ensembl
Negative regulation of proteolysis involved in cellular protein catabolic process Source: ARUK-UCL
Negative regulation of thymocyte apoptotic process Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Notochord formation Source: Ensembl
Positive regulation of amyloid-beta formation Source: ARUK-UCL
Positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process Source: ARUK-UCL
Positive regulation of MAPK cascade Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of protein phosphorylation Source: ARUK-UCL
Positive regulation of protein tyrosine kinase activity Source: ARUK-UCL
Protein stabilization Source: ARUK-UCL
Regulation of angiogenesis Source: Ensembl
Regulation of axonogenesis Source: Ensembl
Regulation of blood vessel endothelial cell migration Source: Ensembl
Regulation of cell adhesion mediated by integrin Source: UniProtKB
Regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Substrate adhesion-dependent cell spreading Source: UniProtKB
Cellular Location
Cell membrane
Ephrin-A1, secreted form: Secreted
PTM
Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form.
N-Glycosylation is required for binding to EPHA2 receptor and inducing its internalization.

Chu, L. Y., Huang, B. L., Huang, X. C., Peng, Y. H., Xie, J. J., & Xu, Y. W. (2022). EFNA1 in gastrointestinal cancer: Expression, regulation and clinical significance. World Journal of Gastrointestinal Oncology, 14(5), 973.

Shen, X., Li, M., Lei, Y., Lu, S., Wang, S., Liu, Z., ... & Zhu, G. (2022). An integrated analysis of single-cell and bulk transcriptomics reveals EFNA1 as a novel prognostic biomarker for cervical cancer. Human Cell, 35(2), 705-720.

Zhang, Y., Zhang, J., Pan, G., Guan, T., Zhang, C., Hao, A., ... & Ren, H. (2021). Effects of EFNA1 on cell phenotype and prognosis of esophageal carcinoma. World journal of surgical oncology, 19(1), 1-10.

Hao, Y. P., Wang, W. Y., Qiao, Q., & Li, G. (2021). EFNA1 is a potential key gene that correlates with immune infiltration in low-grade glioma. Medicine, 100(22).

Mamoor, S. (2021). Differential expression of EFNA1 in cancers of the breast.

Hao, Y., & Li, G. (2020). Role of EFNA1 in tumorigenesis and prospects for cancer therapy. Biomedicine & Pharmacotherapy, 130, 110567.

Asadian, F., Ghadyani, M., Antikchi, M. H., Dastgheib, S. A., Neamatzadeh, H., Sheikhpour, E., ... & Tabei, S. S. (2020). Association of Epidermal Growth Factor 61A> G, Survivin-31G> C, and EFNA1-1732G> A Polymorphisms with Susceptibility to Colorectal Cancer. Journal of Gastrointestinal Cancer, 1-6.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human EFNA1 ELISA Kit (CAT#: V2LY-0626-LY4554)

Human Recombinant EFNA1 protein, hFc Tag (CAT#: V2LY-0526-LY3925)

Human Recombinant EFNA1 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY3926)

Human Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY3927)

Mouse Recombinant EFNA1 protein, hFc Tag (CAT#: V2LY-0526-LY8269)

Mouse Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY8270)

Rat Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY9365)

Related Products

Mouse Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY8270)

Mouse Anti-EFNA1 Recombinant Antibody (EG1037) (CAT#: CBMAB-EN1222-LY)

Mouse Recombinant EFNA1 protein, hFc Tag (CAT#: V2LY-0526-LY8269)

Rat Recombinant EFNA1 protein, His Tag (CAT#: V2LY-0526-LY9365)

Rabbit Anti-EFNA1 Recombinant Antibody (453) (CAT#: V2LY-0425-LY1500)

Mouse Anti-EFNA1 Recombinant Antibody (CBFYE-0484) (CAT#: CBMAB-E0832-FY)

Mouse Anti-EFNA1 Recombinant Antibody (CBFYE-0483) (CAT#: V2LY-0425-LY1502)

Human Recombinant EFNA1 protein, hFc Tag (CAT#: V2LY-0526-LY3925)

Mouse Anti-EFNA1 Recombinant Antibody (1177) (CAT#: V2LY-0425-LY1499)

Human Recombinant EFNA1 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY3926)

Isotype control

For research use only. Not intended for any clinical use.

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