Human Recombinant F11 protein, His Tag (V2LY-0526-LY3994)

Name: Human Recombinant F11 protein, His Tag
SKU: V2LY-0526-LY3994
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant F11 protein, His Tag consist of Amino Acid: 1-625 and predicts a molecular mass of 69.5 kDa.

Molecule Mass

69.5 kDa

Verified

HPLC

Sequence

Amino Acid: 1-625

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥85% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Coagulation Factor XI

Research Area

Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.

Biological Process

Blood coagulation Source: BHF-UCL
Plasminogen activation Source: BHF-UCL
Positive regulation of fibrinolysis Source: BHF-UCL

Cellular Location

Secreted

Involvement in disease

Factor XI deficiency (FA11D):
A hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate.

PTM

N-glycosylated on both chains. N-glycosylated sites mainly consist of nonfucosylated sialylated biantennary (in high abundance) and/or triantennary (in low abundance) complex structures. Glycosylation at Asn-163 uses a rare non-canonical Asn-X-Cys glycosite.
Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.

Wang, H., Jiang, S., Xie, H., Yang, L., Jin, Y., & Wang, M. (2022). Genetic analysis of compound heterozygous pathogenic variants of the F11 gene in two Chinese patients with hereditary factor XI deficiency. Blood Coagulation & Fibrinolysis, 33(1), 61-66.

Cao, Y., Wang, Y., Zhou, Z., Pan, C., Jiang, L., Zhou, Z., ... & Lusis, A. J. (2022). Liver-heart cross-talk mediated by coagulation factor XI protects against heart failure. Science, 377(6613), 1399-1406.

Yang, T., Zhu, J., Chen, X., Wang, F., Zheng, X., & Cheng, X. (2022). Detection and gene mutation analysis of three variations in two unrelated Chinese hereditary coagulation factor XI deficiency families. Acta Haematologica, 1-8.

Yang, T., Zhu, J., Yang, Q., Liu, J., Yang, L., & Wang, M. (2021). Analysis of a pedigree affected with hereditary coagulation factor XI deficiency due to compound heterozygous variants of F11 gene. Zhonghua yi xue yi Chuan xue za zhi= Zhonghua Yixue Yichuanxue Zazhi= Chinese Journal of Medical Genetics, 38(3), 242-246.

Hayakawa, Y., Tamura, S., Suzuki, N., Odaira, K., Tokoro, M., Kawashima, F., ... & Kojima, T. (2021). Essential role of a carboxyl‐terminal α‐helix motif in the secretion of coagulation factor XI. Journal of Thrombosis and Haemostasis, 19(4), 920-930.

Leung, P. Y., Li, C. K., Cheng, C. K., Ng, M. H., & Chan, N. C. (2020). A novel F11 mutation in a Chinese paediatric patient with severe factor XI deficiency. Thrombosis Research, 190, 89-90.

Ciampa, E. J., Liu, N., Stiles, J., Carani, J. L., Li, Y., & Hess, P. E. (2020). Heterozygote carriers of mutations in the F11 gene, encoding Factor XI, have normal coagulation by thromboelastography during pregnancy. International Journal of Obstetric Anesthesia, 42, 57-60.

Liu, M., Li, X., Zhou, X., Jin, Y., Yang, L., Pan, J., ... & Wang, M. (2019). Identification of compound heterozygous mutations of F11 gene in a pedigree affected with heriditary coagulation factor XI deficiency. Zhonghua yi xue yi Chuan xue za zhi= Zhonghua Yixue Yichuanxue Zazhi= Chinese Journal of Medical Genetics, 36(4), 363-367.

Colakoglu, S., Bayhan, T., Tavil, B., Keskin, E. Y., Cakir, V., Gümrük, F., ... & Berber, E. (2018). Molecular genetic analysis of the F11 gene in 14 Turkish patients with factor XI deficiency: identification of novel and recurrent mutations and their inheritance within families. Blood Transfusion, 16(1), 105.

Mohammed, B. M., Cheng, Q., Matafonov, A., Monroe, D. M., Meijers, J. C., & Gailani, D. (2018). Factor XI promotes hemostasis in factor IX‐deficient mice. Journal of Thrombosis and Haemostasis, 16(10), 2044-2049.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

F11 Matched Antibody Pair (392) (CAT#: APMAB-392LY)

Antibody Pairs

F11 Matched Antibody Pair (392) (CAT#: APMAB-392LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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