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Human Recombinant FCER1A protein, ECD, His Tag-1 (V2LY-0526-LY4032)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant FCER1A protein, ECD, His Tag consist of Amino Acid: 1-205 and predicts a molecular mass of 22.5 kDa.
Molecule Mass
22.5 kDa
Protein Domain
ECD
Verified
HPLC
Sequence
Amino Acid: 1-205
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.
Endotoxin
<0.5 EU/mg protein.
Format
Liquid
Buffer
PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Fc Fragment Of IgE Receptor Ia
Research Area
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
Biological Process
Cell surface receptor signaling pathway Source: GO_Central
Regulation of immune response Source: GO_Central
Cellular Location
Cell membrane
Topology
Extracellular: 26-205
Helical: 206-224
Cytoplasmic: 225-257

Tang, R. Y., Yin, L., Yao, L., & Chen, X. P. (2022). An overlapped natural antisense lncRNA FCER1A-AS controls anaphylactic reaction by promoting FcεRIα expression. bioRxiv.

Romantowski, J., Maciejewska, A., Polanska, J., Wasilewska, E., Specjalski, K., Chełminska, M., ... & Niedoszytko, M. (2021). IFNG, FCER1A, PCDHB10 expression as a new potential marker of efficacy in grass pollen allergen-specific immunotherapy. Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii, 38(4), 665.

Andiappan, A. K., Puan, K. J., Lee, B., Yeow, P. T., Yusof, N., Merid, S. K., ... & Rotzschke, O. (2021). Inverse association of FCER1A allergy variant in monocytes and plasmacytoid dendritic cells. Journal of Allergy and Clinical Immunology, 147(4), 1510-1513.

Potaczek, D. P., Przytulska-Szczerbik, A., Bazan-Socha, S., Nastałek, M., Wojas-Pelc, A., Okumura, K., ... & Wypasek, E. (2020). Interaction between functional polymorphisms in FCER1A and TLR2 and the severity of atopic dermatitis. Human Immunology, 81(12), 709-713.

Homma, T., Yokoyama, K., Itoh, F., Sakamoto, T., Yamagishi, M., Ra, C., ... & Inada, Y. (2020). Evaluation of the high affinity IgE receptor alpha-IgG1 Fc fusion protein (FCER1A-Fc) as a topical ocular anti-allergic agent. Journal of Allergy and Clinical Immunology, 145(2), AB162.

Wei, L., Pavlovic, V., Bansal, A. T., Chen, X., Foster, G. R., He, H., ... & Wat, C. (2019). Genetic variation in FCER1A predicts peginterferon alfa‐2a‐induced hepatitis B surface antigen clearance in East Asian patients with chronic hepatitis B. Journal of Viral Hepatitis, 26(9), 1040-1049.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human FCER1A ELISA Kit (CAT#: V2LY-0626-LY4670)

FCER1A Matched Antibody Pair (415) (CAT#: APMAB-415LY)

FCER1A Matched Antibody Pair (416) (CAT#: APMAB-416LY)

Human Recombinant FCER1A proteinECD (CAT#: V2LY-0526-LY4030)

Human Recombinant FCER1A protein, ECD, His Tag (CAT#: V2LY-0526-LY4031)

Human Recombinant FCER1A protein, hFc & AVI Tag (CAT#: V2LY-0526-LY4033)

Mouse Recombinant FCER1A protein, hFc Tag (CAT#: V2LY-0526-LY8301)

Antibody Pairs

FCER1A Matched Antibody Pair (415) (CAT#: APMAB-415LY)

FCER1A Matched Antibody Pair (416) (CAT#: APMAB-416LY)

Related Products

Human Recombinant FCER1A proteinECD (CAT#: V2LY-0526-LY4030)

Mouse Anti-FCER1A Recombinant Antibody (CBXF-3512) (CAT#: V2LY-0925-LY560)

Mouse Anti-FCER1A Recombinant Antibody (C1) (CAT#: CBMAB-F0525-CQ)

Mouse Anti-FCER1A Recombinant Antibody (CBXF-2580) (CAT#: V2LY-0925-LY562)

FCER1A Matched Antibody Pair (415) (CAT#: APMAB-415LY)

Human Recombinant FCER1A protein, ECD, His Tag (CAT#: V2LY-0526-LY4031)

FCER1A Matched Antibody Pair (416) (CAT#: APMAB-416LY)

Human Recombinant FCER1A protein, hFc & AVI Tag (CAT#: V2LY-0526-LY4033)

Mouse Anti-FCER1A Recombinant Antibody (CBXF-0440) (CAT#: CBMAB-F2259-CQ)

Mouse Anti-FCER1A Recombinant Antibody (CBT4088) (CAT#: V2LY-0625-LY921)

Isotype control

For research use only. Not intended for any clinical use.

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