Human Recombinant FRZB protein, His Tag (V2LY-0526-LY4213)

Name: Human Recombinant FRZB protein, His Tag
SKU: V2LY-0526-LY4213
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant FRZB protein, His Tag consist of Amino Acid: 32-325 and predicts a molecular mass of 34.5 kDa.

Molecule Mass

34.5 kDa

Sequence

Amino Acid: 32-325

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

frizzled-related protein

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.

Biological Process

Canonical Wnt signaling pathway Source: GO_Central
Cochlea morphogenesis Source: Ensembl
Convergent extension involved in organogenesis Source: Ensembl
Negative regulation of canonical Wnt signaling pathway Source: UniProtKB
Negative regulation of cartilage development Source: Ensembl
Negative regulation of cell development Source: Ensembl
Negative regulation of cell growth Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of hepatocyte differentiation Source: Ensembl
Negative regulation of Wnt signaling pathway Source: UniProtKB
Neural crest cell differentiation Source: Ensembl
Positive regulation of apoptotic process Source: MGI
Positive regulation of fat cell differentiation Source: MGI
Skeletal system development Source: ProtInc
Somite development Source: Ensembl

Cellular Location

Secreted

Involvement in disease

Osteoarthritis 1 (OS1):
A degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement.

Tuerlings, M., van Hoolwerff, M., van Bokkum, J. M., Suchiman, H. E. D., Lakenberg, N., Broekhuis, D., ... & Meulenbelt, I. (2022). Long non-coding RNA expression profiling of subchondral bone reveals AC005165. 1 modifying FRZB expression during osteoarthritis. Rheumatology, 61(7), 3023-3032.

Xu, R., Zhang, F., Lu, J., Wang, K., Pan, P., Sun, Y., & Zhang, Y. (2021). Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip. Aging (Albany NY), 13(8), 11281.

Huang, X., Zhong, L., van Helvoort, E., Lafeber, F., Mastbergen, S., Hendriks, J., ... & Karperien, M. (2021). The expressions of Dickkopf-related protein 1 and frizzled-related protein are negatively correlated to local inflammation and osteoarthritis severity. Cartilage, 12(4), 496-504.

Casas-Fraile, L., Cornelis, F. M., Costamagna, D., Rico, A., Duelen, R., Sampaolesi, M. M., ... & Sáenz, A. (2020). Frizzled related protein deficiency impairs muscle strength, gait and calpain 3 levels. Orphanet Journal of Rare Diseases, 15(1), 1-18.

Kwan, T., Kazamel, M., Thoenes, K., Si, Y., Jiang, N., & King, P. H. (2020). Wnt antagonist FRZB is a muscle biomarker of denervation atrophy in amyotrophic lateral sclerosis. Scientific reports, 10(1), 16679.

Claudel, M., Jouzeau, J. Y., & Cailotto, F. (2019). Secreted Frizzled‐related proteins (sFRPs) in osteo‐articular diseases: much more than simple antagonists of Wnt signaling?. The FEBS Journal, 286(24), 4832-4851.

Casas-Fraile, L., Cornelis, F. M., Costamagna, D., Rico, A., Sampaolesi, M., de Munain, A. L., ... & Lories, R. J. (2018). Effect of frizzled related protein (FRZB) on muscles: an inverse relationship between FRZB and calpain-3 with potential impact on muscle dystrophy and osteoarthritis. Osteoarthritis and Cartilage, 26, S401.

Huang, X., Post, J. N., Zhong, L., Leijten, J., Larsson, S., Karperien, M., & Struglics, A. (2018). Dickkopf-related protein 1 and gremlin 1 show different response than frizzled-related protein in human synovial fluid following knee injury and in patients with osteoarthritis. Osteoarthritis and cartilage, 26(6), 834-843.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human FRZB ELISA Kit (CAT#: V2LY-0626-LY3989)

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Isotype control

For research use only. Not intended for any clinical use.

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