Human Recombinant GABARAPL2 protein, His Tag (V2LY-0526-LY4234)

Name: Human Recombinant GABARAPL2 protein, His Tag
SKU: V2LY-0526-LY4234
Rating: 5 (1 reviews)

See all GABARAPL2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GABARAPL2 protein, His Tag consist of Amino Acid: 1-117 and predicts a molecular mass of 15.5 kDa.

Molecule Mass

15.5 kDa

Sequence

Amino Acid: 1-117

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

GABA type A receptor associated protein like 2

Function

Ubiquitin-like modifier involved in intra-Golgi traffic (By similarity).

Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation (By similarity).

It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity).

Involved in autophagy (PubMed:20418806, PubMed:23209295).

Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:20418806, PubMed:23209295).

Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295).

Biological Process

Autophagosome assembly Source: GO_Central
Autophagy Source: UniProtKB
Autophagy of mitochondrion Source: GO_Central
Cellular response to nitrogen starvation Source: GO_Central
Intra-Golgi vesicle-mediated transport Source: UniProtKB
Macroautophagy Source: GO_Central
Negative regulation of proteasomal protein catabolic process Source: BHF-UCL
Positive regulation of ATPase activity Source: UniProtKB
Protein localization to endoplasmic reticulum Source: UniProtKB
Protein transport Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum membrane; Golgi apparatus; Autophagosome

PTM

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant GABARAPL2 protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human GABARAPL2 ELISA Kit (CAT#: V2LY-0626-LY4605)

Related Products

Rabbit Anti-GABARAPL2 Recombinant Antibody (1W9) (CAT#: V2LY-1225-LY499)

Rabbit Anti-GABARAPL2 Recombinant Antibody (D1W9T) (CAT#: CBMAB-G1605-LY)

Human GABARAPL2 ELISA Kit (CAT#: V2LY-0626-LY4605)

Rat Anti-GABARAPL2 Recombinant Antibody (853746) (CAT#: CBMAB-G0115-LY)

Rabbit Anti-GABARAPL2 Recombinant Antibody (46) (CAT#: V2LY-1225-LY498)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry