Human Recombinant GCGR protein, His Tag (V2LY-0526-LY4274)

Name: Human Recombinant GCGR protein, His Tag
SKU: V2LY-0526-LY4274
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GCGR protein, His Tag consist of Amino Acid: 1-136 and predicts a molecular mass of 14.5 kDa.

Molecule Mass

14.5 kDa

Verified

HPLC

Sequence

Amino Acid: 1-136

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, EDTA, Flagtide, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

glucagon receptor

Function

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: UniProtKB
Cell surface receptor signaling pathway Source: InterPro
Cellular response to glucagon stimulus Source: UniProtKB
Cellular response to starvation Source: ARUK-UCL
Exocytosis Source: Ensembl
Generation of precursor metabolites and energy Source: ProtInc
Glucose homeostasis Source: UniProtKB
Hormone-mediated signaling pathway Source: Ensembl
Positive regulation of gene expression Source: ARUK-UCL
Regulation of blood pressure Source: ProtInc
Regulation of glycogen metabolic process Source: UniProtKB
Response to nutrient Source: ProtInc
Response to starvation Source: UniProtKB

Cellular Location

Cell membrane. Is rapidly internalized after ligand-binding.

Topology

Extracellular: 26-136
Helical: 137-161
Cytoplasmic: 162-173
Helical: 174-198
Extracellular: 199-225
Helical: 226-249
Cytoplasmic: 250-263
Helical: 264-285
Extracellular: 286-303
Helical: 304-326
Cytoplasmic: 327-350
Helical: 351-369
Extracellular: 370-381
Helical: 382-402
Cytoplasmic: 403-477

PTM

Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant GCGR (Nanodisc) protein, His Tag (CAT#: V2LY-0526-LY4275)

Human Recombinant GCGR-VLP protein (CAT#: V2LY-0526-LY4276)

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Human Recombinant GCGR (Nanodisc) protein, His Tag (CAT#: V2LY-0526-LY4275)

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Human Anti-GCGR Recombinant Antibody (19E8) (CAT#: V2LY-1225-LY892)

Human Recombinant GCGR-VLP protein (CAT#: V2LY-0526-LY4276)

Rat Anti-GCGR Recombinant Antibody (EG1332) (CAT#: CBMAB-EN1610-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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