Human Recombinant GM2A protein, His Tag (V2LY-0526-LY4356)

Name: Human Recombinant GM2A protein, His Tag
SKU: V2LY-0526-LY4356
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GM2A protein, His Tag consist of Amino Acid: 1-193 and predicts a molecular mass of 19.8 kDa.

Molecule Mass

19.8 kDa

Sequence

Amino Acid: 1-193

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>96% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

GM2 Ganglioside Activator

Function

The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity).

Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3 (By similarity).

Has cholesterol transfer activity (PubMed:17552909).

Biological Process

Ganglioside catabolic process Source: GO_Central
Glycosphingolipid metabolic process Source: Reactome
Learning or memory Source: Ensembl
Lipid storage Source: Ensembl
Lipid transport Source: GO_Central
Neuromuscular process controlling balance Source: Ensembl
Oligosaccharide catabolic process Source: Ensembl
Positive regulation of hydrolase activity Source: Ensembl

Cellular Location

Lysosome

Involvement in disease

GM2-gangliosidosis AB (GM2GAB):
An autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. It is characterized by GM2 gangliosides accumulation in the presence of both normal hexosaminidase A and B.

PTM

The serines in positions 32 and 33 are absent in 80% of the sequenced protein.

Hsieh, Y. C., Negri, J., He, A., Pearse, R. V., Liu, L., Duong, D. M., ... & Young-Pearse, T. L. (2022). Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity. Molecular neurodegeneration, 17(1), 61.

Xiao, C., Toro, C., & Tifft, C. (2022). GM2 Activator Deficiency. GeneReviews®[Internet].

Kafaei Shahbaz, N. (2022). The Role of GM2 Activator Protein in Breast Cancer Metastasis and the EGFR Signaling Pathway (Doctoral dissertation).

Yuan, J., Li, Z., Lin, Z., Yao, S., Han, Y., Fu, Q., & Liu, J. (2022). Label-free quantitative proteomics reveals the Steap3-Gm2a axis inhibiting the phagosomal escape of Listeria monocytogenes. Microbes and Infection, 24(8), 104999.

Beeler, K., Vowinckel, J., Soste, M., Mallardo, D., Capone, M., Gabriele, M., ... & Ascierto, P. (2020). 229 Discovery of ganglioside GM2 activator as a novel proteomic biomarker associated with response to treatment in first-line melanoma subjects treated with PD-1 immunotherapy.

Holzer, K., Drucker, E., Waldburger, N., Eiteneuer, E., Breuhahn, K., Ori, A., ... & Singer, S. (2018). Ganglioside GM2 activator (GM2A) is as a novel pro-survival p53 target in hepatocellular carcinoma. Zeitschrift für Gastroenterologie, 56(01), A4-44.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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