Human Recombinant GNAI3, Active protein, GST Tag (V2LY-0526-LY4374)

Name: Human Recombinant GNAI3, Active protein, GST Tag
SKU: V2LY-0526-LY4374
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant GNAI3, Active protein, GST Tag consist of Amino Acid: 2-end and predicts a molecular mass of 65 kDa.

Molecule Mass

65 kDa

Sequence

Amino Acid: 2-end

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

G protein subunit alpha i3

Function

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087).

Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087).

Stimulates the activity of receptor-regulated K+ channels (PubMed:2535845).

The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).

Biological Process

Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: UniProtKB
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Brain development Source: Ensembl
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB
Dopamine receptor signaling pathway Source: UniProtKB
GTP metabolic process Source: UniProtKB
Negative regulation of adenylate cyclase activity Source: ProtInc
Negative regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of macroautophagy Source: UniProtKB
Positive regulation of NAD(P)H oxidase activity Source: Ensembl
positive regulation of superoxide anion generation Source: Ensembl
Positive regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
Vesicle fusion Source: Ensembl

Cellular Location

Cell membrane; Cytoplasm; Centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.

Involvement in disease

Auriculocondylar syndrome 1 (ARCND1):
An autosomal dominant craniofacial malformation syndrome characterized by variable mandibular anomalies, including mild to severe micrognathia, temporomandibular joint ankylosis, cleft palate, and a characteristic ear malformation that consists of separation of the lobule from the external ear, giving the appearance of a question mark (question-mark ear). Other frequently described features include prominent cheeks, cupped and posteriorly rotated ears, preauricular tags, and microstomia.

PTM

(Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.

Leiss, V., Reisinger, E., Speidel, A., Beer-Hammer, S., & Nürnberg, B. (2021). Analyses of Gnai3-iresGFP reporter mice reveal unknown Gαi3 expression sites. Scientific Reports, 11(1), 14271.

Mishra, C., & Mohapatra, S. (2020). An integrative bioinformatics analysis of caprine GNAI3 gene. Journal of Genetics, 99, 1-7.

Li, Z. W., Sun, B., Gong, T., Guo, S., Zhang, J., Wang, J., ... & Chu, W. M. (2019). GNAI1 and GNAI3 reduce colitis-associated tumorigenesis in mice by blocking IL6 signaling and down-regulating expression of GNAI2. Gastroenterology, 156(8), 2297-2312.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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