Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (V2LY-0226-LY147)
Compare
Online Inquiry
Published Data
Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (V2LY-0226-LY147) in WB
Expression of Gai1, Gai3, and GAPDH in diseased nasal mucosa in patients with CRSsNP and those with eCRSwNP and non-eCRSwNP was tested by western blotting.
...View More
Xie, H., Ji, J., Liu, Z., Lu, N., Wei, Y., Zhou, A., Liu, J., & Jiao, Q. (2025). Gαi1/3 signaling mediates IL-5-induced eosinophil activation and type 2 inflammation in eosinophilic chronic rhinosinusitis. Frontiers in immunology, 15, 1460104. https://doi.org/10.3389/fimmu.2024.1460104
Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (V2LY-0226-LY147) in IHC
Gai1/3 IHC of patients with CRSsNP and those with eCRSwNP and non-eCRSwNP.
...View More
Xie, H., Ji, J., Liu, Z., Lu, N., Wei, Y., Zhou, A., Liu, J., & Jiao, Q. (2025). Gαi1/3 signaling mediates IL-5-induced eosinophil activation and type 2 inflammation in eosinophilic chronic rhinosinusitis. Frontiers in immunology, 15, 1460104. https://doi.org/10.3389/fimmu.2024.1460104
Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (V2LY-0226-LY147) in WB
Relative expression of Gαi/o subunits, main 2-AG-related enzymes, and synaptic proteins in hippocampal synaptosomes (Syn). (A) Representative Western blots carried out by immunoblotting increasing amounts of hippocampal synaptosomes. The total protein loading was checked by Coomassie Brilliant Blue gel staining. Protein migration was consistent with their expected molecular mass (PLCβ1, 138.3 kDa; DAGLα, 115.3 kDa; DAGLβ, 73.9 kDa; MAGL, 33.3 kDa; Gαo 40.1 kDa; Gαi1, 40.5 kDa; Gαi2, 40.4 kDa; Gαi3, 40.5 kDa; PSD-95, 80.4 kDa; SNAP-25, 23.3 kDa). The molecular weights depicted correspond to the signal of the standard markers. (B) Gαo, Gαi1, Gαi2, and Gαi3 expression in synaptosomes. (C) PLCβ1, DAGLα, DAGLβ, and MAGL expression in synaptosomes. (D) SNAP-25 and PSD-95 expression in synaptosomes. Data are presented as mean ± SEM (see Table 4) with squares representing individual experimental values, using synaptosome membranes obtained from two fractionation procedures and including hippocampal pools from at least six adult mice per fractionation procedure. Statistical analysis: Paired ratio t-test; * p < 0.05, ** p < 0.01, *** p < 0.001.
...View More
Serrano, M., Saumell-Esnaola, M., Ocerin, G., García Del Caño, G., Puente, N., Sallés, J., Rodríguez de Fonseca, F., Rodríguez-Arias, M., Gerrikagoitia, I., & Grandes, P. (2024). Impact of Omega-3 on Endocannabinoid System Expression and Function, Enhancing Cognition and Behavior in Male Mice. Nutrients, 16(24), 4344. https://doi.org/10.3390/nu16244344
Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (V2LY-0226-LY147) in WB
The 92.1 cells were engineered to stably express the lentiviral PCK1 shRNA ("shPCK1-seq-1" or "shPCK1-seq-2", the lentiviral control shRNA ("shC") (A-C), the lentiviral CRISPR/Cas9-PCK1-KO construct ("koPCK1"), the CRISPR/Cas9-control construct ("Cas9-C") (D, E), the lentivirus-packed PCK1-expressing construct ("oePCK1") or the empty vector ("Vec") (F, G), the mRNA and protein expression of Gαi3 was tested (A, B, D-G). ChIP assay showed the relative levels of Gαi3 promoter DNA binding to GATA4 in shC cells and PCK1-silenced cells (C). The shPCK1-seq-2-expressing 92.1 cells were subjected to stable transduction with the lentivirus-packed Gαi3-expressing construct ("oeGαi3") or an empty vector ("Vec"). The expression levels of the listed proteins were shown (H). An equal number of the aforementioned 92.1 cells were cultured for specific durations, cell proliferation, migration, and apoptosis were assessed using EdU-nuclei staining (I), "Transwell" assays (J), and TUNEL-nuclei staining (K), respectively, with results quantified. The stable 92.1 UVM cells with the lentiviral Gαi3 shRNA ("shGαi3-seq-1" or "shGαi3-seq-2", two different sequences) or the lentiviral control shRNA ("shC") were established, and expression of listed proteins was shown (L). The numerical values are presented as the mean ± standard deviation (SD). "Pare" signifies the parental control cells. *Indicates statistical significance (P < 0.05) when compared to "shC"/"Cas9-C"/"Vec" cells (A-G and L). #P < 0.05 (H-K). "N. S." denotes a lack of statistical difference (P > 0.05). The experiments depicted in this figure were replicated five times (n = 5, biological repeats), consistently yielding similar results.
...View More
Bi, H. E., Zhang, J., Yao, Y., Wang, S., Yao, J., Shao, Z., & Jiang, Q. (2024). Expression and functional significance of phosphoenolpyruvate carboxykinase 1 in uveal melanoma. Cell death discovery, 10(1), 196. https://doi.org/10.1038/s41420-024-01963-y
Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (V2LY-0226-LY147) in WB
Gαi1 plus Gαi3 double knockout abolishes Netrin-1-induced Akt-mTOR and Erk activation in MEFs. Wild-type (WT) mouse embryonic fibroblasts (MEFs), Gαi1 plus Gαi3 double knockout ("Gαi1/3 DKO") MEFs (A-C), Gαi1, Gαi2 or Gαi3 single knockout (SKO) MEFs (D-E) were treated with Netrin-1 at the applied concentrations for designated time periods, expression of listed proteins was shown, and protein phosphorylation was quantified (A-E). Data were expressed as mean ± standard deviation (SD, biological replicates). Quantifications were from five biological replicates (n = 5). *P < 0.05 versus "WT MEFs". #P < 0.05. "N. S." stands for non-statistical differences (P> 0.05).
...View More
Li, Y., Chai, J. L., Shi, X., Feng, Y., Li, J. J., Zhou, L. N., Cao, C., & Li, K. R. (2023). Gαi1/3 mediate Netrin-1-CD146-activated signaling and angiogenesis. Theranostics, 13(7), 2319-2336. https://doi.org/10.7150/thno.80749
Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (V2LY-0226-LY147) in IF
Confocal immunofluorescence of CREB (red), Actin (green) and DAPI (blue). Scale bar = 20 μm. Confocal immunofluorescence of YAP (red), Actin (green) and DAPI (blue). Scale bar = 20 μm. The experiments were performed in triplicate. The data are presented as means ± SD, and significant differences were evaluated using unpaired t test. *P < 0.05, **P < 0.01, ***P < 0.005 and ns, not significant.
...View More
Yin, S., Song, R., Ma, J., Liu, C., Wu, Z., Cao, G., Liu, J., Zhang, G., Zhang, H., Sun, R., Chen, A., & Wang, Y. (2022). Receptor activity-modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. Cell communication and signaling : CCS, 20(1), 52. https://doi.org/10.1186/s12964-022-00852-0
Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (V2LY-0226-LY147) in IHC
G-protein immunohistochemistry on sections of the olfactory mucosa of Chiloscyllium punctatum. The five antisera against G-protein α-subunits used labeled different olfactory receptor neurons (ORNs) (left column, red fluorescence) and Hoechst stained all nuclei (blue). (First row) antisera against Gαs/olf labeled mainly long ORNs, which soma sits in the lower layers of the neuroepithelium. These elongated cells have a rounded nucleus occupying most of the soma, a long and relatively narrow dendrite ending in an apical knob, which protrusions were not clearly associated with their soma. This marker also labeled other somas in the intermediate to low layers of the neuroepithelium (sometimes with associated cilia above the neuroepithelium surface but no dendrite clearly labeled), and randomly scattered cilia (sectioned or not). Sectioned labeled cilia showed in inset image. (Second row) antisera against Gαq/11/14 labeled mainly microvillous and crypt ORNs (inset image), and partial labeling of ORN dendrites in the upper neuroepithelium. (Third row) antisera against Gαi-1/2/3 labeled intermediate somas and possible associated cilia, some more faint apical cells, as well as progenitor cells in the basal layer (inset image). (Fourth row) antisera against Gαi-3 labeled somas in lower half of the neuroepithelium, possible associated cilia, and ORN axons in the lamina propria. (Fifth row) antisera against Gαo labeled the somas of different microvilli-bearing ORN populations, including the microvillous morphotype, the pear-shaped morphotype with a swelled dendrite, and the teardrop-shaped morphotype, as well as some short (perhaps sectioned) cilia close to the apical membrane, and scarce longer cilia (inset image). White arrowheads, labeled cilia. Scale bars = 10 μm.
...View More
Camilieri-Asch, V., Caddy, H. T., Hubbard, A., Rigby, P., Doyle, B., Shaw, J. A., Mehnert, A., Partridge, J. C., Yopak, K. E., & Collin, S. P. (2020). Multimodal Imaging and Analysis of the Neuroanatomical Organization of the Primary Olfactory Inputs in the Brownbanded Bamboo Shark, Chiloscyllium punctatum. Frontiers in neuroanatomy, 14, 560534. https://doi.org/10.3389/fnana.2020.560534
Datasheet
Target
References
Q & As
Review & reward
Protocols
Associated Products
Basic Information
Host Animal
Mouse
Clone
CBLG1-1409
Application
WB, ELISA, IF, IP
Immunogen
Amino acids 339-354 at the C-terminus of rat Gα i-3.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Horse, Dog, Cattle, Pig, Chicken
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Formulations & Storage [For reference only, actual COA shall prevail!]
Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation
Target
Full Name
G protein subunit alpha i3
Function
Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087).
Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087).
Stimulates the activity of receptor-regulated K+ channels (PubMed:2535845).
The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).
Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087).
Stimulates the activity of receptor-regulated K+ channels (PubMed:2535845).
The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).
Biological Process
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: UniProtKB
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Brain development Source: Ensembl
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB
Dopamine receptor signaling pathway Source: UniProtKB
GTP metabolic process Source: UniProtKB
Negative regulation of adenylate cyclase activity Source: ProtInc
Negative regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of macroautophagy Source: UniProtKB
Positive regulation of NAD(P)H oxidase activity Source: Ensembl
positive regulation of superoxide anion generation Source: Ensembl
Positive regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
Vesicle fusion Source: Ensembl
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Brain development Source: Ensembl
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB
Dopamine receptor signaling pathway Source: UniProtKB
GTP metabolic process Source: UniProtKB
Negative regulation of adenylate cyclase activity Source: ProtInc
Negative regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of macroautophagy Source: UniProtKB
Positive regulation of NAD(P)H oxidase activity Source: Ensembl
positive regulation of superoxide anion generation Source: Ensembl
Positive regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
Vesicle fusion Source: Ensembl
Cellular Location
Cell membrane; Cytoplasm; Centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
Involvement in disease
Auriculocondylar syndrome 1 (ARCND1):
An autosomal dominant craniofacial malformation syndrome characterized by variable mandibular anomalies, including mild to severe micrognathia, temporomandibular joint ankylosis, cleft palate, and a characteristic ear malformation that consists of separation of the lobule from the external ear, giving the appearance of a question mark (question-mark ear). Other frequently described features include prominent cheeks, cupped and posteriorly rotated ears, preauricular tags, and microstomia.
An autosomal dominant craniofacial malformation syndrome characterized by variable mandibular anomalies, including mild to severe micrognathia, temporomandibular joint ankylosis, cleft palate, and a characteristic ear malformation that consists of separation of the lobule from the external ear, giving the appearance of a question mark (question-mark ear). Other frequently described features include prominent cheeks, cupped and posteriorly rotated ears, preauricular tags, and microstomia.
PTM
(Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.
Have you used Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon
$30 eGift Card
Submit a review
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon
$30 eGift Card
Submit a review
Loading...
Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
For research use only. Not intended for any clinical use.
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
Online InquiryContact us
Online Inquiry