Human Recombinant GNLY protein, ECD, hFc Tag (V2LY-0526-LY4404)

Go to compare Compare Online Inquiry
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
hFc Tag
Protein Construction
This product is Human Recombinant GNLY protein, ECD, hFc Tag consist of Amino Acid: 23-145 and predicts a molecular mass of 41.12 kDa.
Molecule Mass
41.12 kDa
Protein Domain
ECD
Sequence
Amino Acid: 23-145
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>90% as determined by SDS-PAGE.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
granulysin
Function
Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.
Biological Process
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Cellular defense response Source: UniProtKB
Defense response to bacterium Source: UniProtKB
Defense response to fungus Source: UniProtKB-KW
Killing of cells of other organism Source: UniProtKB
Cellular Location
Secreted. Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.
PTM
A 9 kDa form is produced by proteolytic processing of a 15 kDa protein.

Thakur, P., Sutiwisesak, R., Lu, Y. J., & Behar, S. M. (2022). Use of the Human Granulysin Transgenic Mice To Evaluate the Role of Granulysin Expression by CD8 T Cells in Immunity To Mycobacterium tuberculosis. Mbio, 13(6), e03020-22.

Milovanović, J., Todorović-Raković, N., Vujasinović, T., Greenman, J., Mandušić, V., & Radulovic, M. (2022). Can granulysin provide prognostic value in primary breast cancer?. Pathology-Research and Practice, 237, 154039.

Drvar, V., Ćurko‑Cofek, B., Karleuša, L., Aralica, M., Rogoznica, M., Kehler, T., ... & Laskarin, G. (2022). Granulysin expression and granulysin‑mediated apoptosis in the peripheral blood of osteoarthritis patients. Biomedical reports, 16(5), 1-10.

Chu, M. T., Wang, C. W., Chang, W. C., Chen, C. B., Chung, W. H., & Hung, S. I. (2021). Granulysin-based lymphocyte activation test for evaluating drug causality in antiepileptics-induced severe cutaneous adverse reactions. Journal of Investigative Dermatology, 141(6), 1461-1472.

Crespo, Â. C., Mulik, S., Dotiwala, F., Ansara, J. A., Santara, S. S., Ingersoll, K., ... & Lieberman, J. (2020). Decidual NK cells transfer granulysin to selectively kill bacteria in trophoblasts. Cell, 182(5), 1125-1139.

Hojo-Souza, N. S., de Azevedo, P. O., de Castro, J. T., Teixeira-Carvalho, A., Lieberman, J., Junqueira, C., & Gazzinelli, R. T. (2020). Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage. PLoS Pathogens, 16(9), e1008840.

Dotiwala, F., & Lieberman, J. (2019). Granulysin: killer lymphocyte safeguard against microbes. Current opinion in immunology, 60, 19-29.

Vičić, M., Kaštelan, M., Tokmadžić, V. S., & Massari, L. P. (2019). Systemic and local increase of granulysin expression in cytotoxic lymphocytes in severe psoriasis. Acta dermato-venereologica, 99(12), 1136-1142.

Lettau, M., Dietz, M., Dohmen, K., Leippe, M., Kabelitz, D., & Janssen, O. (2019). Granulysin species segregate to different lysosome-related effector vesicles (LREV) and get mobilized by either classical or non-classical degranulation. Molecular immunology, 107, 44-53.

Gulic, T., Laskarin, G., Dominovic, M., Glavan Gacanin, L., Babarović, E., Rubesa, Z., ... & Rukavina, D. (2018). Granulysin‐mediated apoptosis of trophoblasts in blighted ovum and missed abortion. American journal of reproductive immunology, 80(3), e12978.

Ask a question We look forward to hearing from you.
0 reviews or Q&As
Loading...
Have you used Human Recombinant GNLY protein, ECD, hFc Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

online inquiry
Online Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.