Human Recombinant GPC5 protein, Biotin Conjugated, AVI Tag (V2LY-0526-LY4354)

Name: Human Recombinant GPC5 protein, Biotin Conjugated, AVI Tag
SKU: V2LY-0526-LY4354
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

AVI Tag

Protein Construction

This product is Human Recombinant GPC5 protein, Biotin Conjugated, AVI Tag consist of Amino Acid: 1-554 and predicts a molecular mass of 62.37 kDa.

Molecule Mass

62.37 kDa

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-554

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC. ≥90% as determined by SEC-MALS(Routinely tested).

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

glypican 5

Function

Cell surface proteoglycan that bears heparan sulfate.

Biological Process

Cell migration Source: GO_Central
Positive regulation of canonical Wnt signaling pathway Source: GO_Central
Regulation of protein localization to membrane Source: GO_Central

Cellular Location

Cell membrane
Secreted glypican-5: Extracellular space

Bosworth, A. P., Contreras, M., Novak, S. W., Sancho, L., Salas, I. H., Manor, U., & Allen, N. J. (2023). Astrocyte glypican 5 regulates synapse maturation and stabilization. bioRxiv, 2023-03.

Takeuchi, M., Takeuchi, K., Takai, T., Yamaguchi, R., Furukawa, T., Akagi, K. I., & Takeuchi, J. K. (2021). Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT. Plos one, 16(2), e0226538.

Schlaepfer Sales, C. B., Guimarães, V. S., Valverde, L. F., Dias, R. B., Freitas, R. D., Siquara da Rocha, L. D. O., ... & Gurgel Rocha, C. A. (2021). Glypican-1,-3,-5 (GPC1, GPC3, GPC5) and Hedgehog Pathway Expression in Oral Squamous Cell Carcinoma. Applied Immunohistochemistry & Molecular Morphology, 29(5), 345-351.

Yang, X., Chen, Y., Zhou, Y., Wu, C., Li, Q., Wu, J., ... & Ji, M. (2021). GPC5 suppresses lung cancer progression and metastasis via intracellular CTDSP1/AhR/ARNT signaling axis and extracellular exosome secretion. Oncogene, 40(25), 4307-4323.

Takeuchi, M., Takeuchi, K., Monobe, Y., Takai, T., Yamaguchi, R., Takeuchi, J. K., & Akagi, K. I. (2019). Subcellular localization of glypican-5 associates with dynamic cell motility and cell communication of the human mesenchymal stem cell line U3DT. bioRxiv, 863720.

Hong, X., Zhang, Z., Pan, L., Ma, W., Zhai, X., Gu, C., ... & Liu, Z. (2019). MicroRNA-301b promotes the proliferation and invasion of glioma cells through enhancing activation of Wnt/β-catenin signaling via targeting Glypican-5. European Journal of Pharmacology, 854, 39-47.

Yuan, Q., Zhang, Y., Li, J., Cao, G., & Yang, W. (2018). High expression of microRNA-4295 contributes to cell proliferation and invasion of pancreatic ductal adenocarcinoma by the down-regulation of Glypican-5. Biochemical and biophysical research communications, 497(1), 73-79.

Sun, Y., Xu, K., He, M., Fan, G., & Lu, H. (2018). Overexpression of glypican 5 (GPC5) inhibits prostate cancer cell proliferation and invasion via suppressing Sp1-mediated EMT and activation of Wnt/β-catenin signaling. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, 26(4), 565-572.

Chui, M. H., Have, C., Hoang, L. N., Shaw, P., Lee, C. H., & Clarke, B. A. (2018). Genomic profiling identifies GPC5 amplification in association with sarcomatous transformation in a subset of uterine carcinosarcomas. The Journal of Pathology: Clinical Research, 4(1), 69-78.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant GPC5 protein, His Tag (CAT#: V2LY-0526-LY4353)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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