Human Recombinant GZMB protein, His Tag (V2LY-0526-LY4406)

Name: Human Recombinant GZMB protein, His Tag
SKU: V2LY-0526-LY4406
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GZMB protein, His Tag consist of Amino Acid: 1-247 and predicts a molecular mass of 27.2 kDa.

Molecule Mass

27.2 kDa

Verified

HPLC

Sequence

Amino Acid: 1-247

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥97% as determined by SDS-PAGE ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Granzyme B

Function

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3262682, PubMed:3263427, PubMed:1985927).

It cleaves after Asp (PubMed:8258716, PubMed:1985927).

Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:32188940, PubMed:31953257).

Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed:9852092).

Biological Process

Apoptotic process Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Granzyme-mediated programmed cell death signaling pathway Source: UniProtKB
Natural killer cell mediated cytotoxicity Source: UniProtKB
Negative regulation of translation Source: CACAO
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Proteolysis involved in cellular protein catabolic process Source: UniProtKB
Pyroptosis Source: UniProtKB

Cellular Location

Cytolytic granule; Secreted. Delivered into the target cell by perforin (PubMed:20038786).

Gleave, A., & Granville, D. J. (2023). Granzyme B in Autoimmune Skin Disease. Biomolecules, 13(2), 388.

Tibbs, E., & Cao, X. (2022). Emerging canonical and non-canonical roles of granzyme B in health and disease. Cancers, 14(6), 1436.

Wang, H., Huang, Y., He, J., Zhong, L., & Zhao, Y. (2021). Dual roles of granzyme B. Scandinavian Journal of Immunology, 94(3), e13086.

Turner, C. T., Zeglinski, M. R., Richardson, K. C., Santacruz, S., Hiroyasu, S., Wang, C., ... & Granville, D. J. (2021). Granzyme B contributes to barrier dysfunction in oxazolone-induced skin inflammation through E-cadherin and FLG cleavage. Journal of Investigative Dermatology, 141(1), 36-47.

Turner, C. T., Bolsoni, J., Zeglinski, M. R., Zhao, H., Ponomarev, T., Richardson, K., ... & Granville, D. J. (2021). Granzyme B mediates impaired healing of pressure injuries in aged skin. npj Aging and Mechanisms of Disease, 7(1), 6.

Xie, M. M., Fang, S., Chen, Q., Liu, H., Wan, J., & Dent, A. L. (2019). Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells. JCI insight, 4(16).

Turner, C. T., Lim, D., & Granville, D. J. (2019). Granzyme B in skin inflammation and disease. Matrix Biology, 75, 126-140.

Arabpour, M., Rasolmali, R., Talei, A. R., Mehdipour, F., & Ghaderi, A. (2019). Granzyme B production by activated B cells derived from breast cancer-draining lymph nodes. Molecular immunology, 114, 172-178.

Chaves-Pozo, E., Valero, Y., Lozano, M. T., Rodríguez-Cerezo, P., Miao, L., Campo, V., ... & Cuesta, A. (2019). Fish granzyme A shows a greater role than granzyme B in fish innate cell-mediated cytotoxicity. Frontiers in Immunology, 10, 2579.

Russo, V., Klein, T., Lim, D. J., Solis, N., Machado, Y., Hiroyasu, S., ... & Granville, D. J. (2018). Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction. Scientific reports, 8(1), 9690.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant GZMB protein, hFc Tag (CAT#: V2LY-0526-LY4405)

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Isotype control

For research use only. Not intended for any clinical use.

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