Rat Gzmb ELISA Kit (V2LY-0626-LY848)

Name: Rat Gzmb ELISA Kit
SKU: V2LY-0626-LY848
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0033 ng/mL

Detection Range

0.007-1.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Rat

Assay Type

Sandwich

Reactivity

Rat

Assay Time

1.5 h

Molecule Mass

27.3 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Granzyme B

Function

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3262682, PubMed:3263427, PubMed:1985927).

It cleaves after Asp (PubMed:8258716, PubMed:1985927).

Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:32188940, PubMed:31953257).

Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed:9852092).

Biological Process

Apoptotic process Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Granzyme-mediated programmed cell death signaling pathway Source: UniProtKB
Natural killer cell mediated cytotoxicity Source: UniProtKB
Negative regulation of translation Source: CACAO
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Proteolysis involved in cellular protein catabolic process Source: UniProtKB
Pyroptosis Source: UniProtKB

Cellular Location

Cytolytic granule; Secreted. Delivered into the target cell by perforin (PubMed:20038786).

Gleave, A., & Granville, D. J. (2023). Granzyme B in Autoimmune Skin Disease. Biomolecules, 13(2), 388.

Tibbs, E., & Cao, X. (2022). Emerging canonical and non-canonical roles of granzyme B in health and disease. Cancers, 14(6), 1436.

Wang, H., Huang, Y., He, J., Zhong, L., & Zhao, Y. (2021). Dual roles of granzyme B. Scandinavian Journal of Immunology, 94(3), e13086.

Turner, C. T., Zeglinski, M. R., Richardson, K. C., Santacruz, S., Hiroyasu, S., Wang, C., ... & Granville, D. J. (2021). Granzyme B contributes to barrier dysfunction in oxazolone-induced skin inflammation through E-cadherin and FLG cleavage. Journal of Investigative Dermatology, 141(1), 36-47.

Turner, C. T., Bolsoni, J., Zeglinski, M. R., Zhao, H., Ponomarev, T., Richardson, K., ... & Granville, D. J. (2021). Granzyme B mediates impaired healing of pressure injuries in aged skin. npj Aging and Mechanisms of Disease, 7(1), 6.

Xie, M. M., Fang, S., Chen, Q., Liu, H., Wan, J., & Dent, A. L. (2019). Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells. JCI insight, 4(16).

Turner, C. T., Lim, D., & Granville, D. J. (2019). Granzyme B in skin inflammation and disease. Matrix Biology, 75, 126-140.

Arabpour, M., Rasolmali, R., Talei, A. R., Mehdipour, F., & Ghaderi, A. (2019). Granzyme B production by activated B cells derived from breast cancer-draining lymph nodes. Molecular immunology, 114, 172-178.

Chaves-Pozo, E., Valero, Y., Lozano, M. T., Rodríguez-Cerezo, P., Miao, L., Campo, V., ... & Cuesta, A. (2019). Fish granzyme A shows a greater role than granzyme B in fish innate cell-mediated cytotoxicity. Frontiers in Immunology, 10, 2579.

Russo, V., Klein, T., Lim, D. J., Solis, N., Machado, Y., Hiroyasu, S., ... & Granville, D. J. (2018). Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction. Scientific reports, 8(1), 9690.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant GZMB protein, hFc Tag (CAT#: V2LY-0526-LY4405)

Human Recombinant GZMB protein, His Tag (CAT#: V2LY-0526-LY4406)

Human GZMB ELISA Kit (CAT#: V2LY-0626-LY1244)

Mouse Gzmb ELISA Kit (CAT#: V2LY-0626-LY878)

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Mouse Anti-GZMB Recombinant Antibody (27) (CAT#: CBMAB-G0993-LY)

Mouse Anti-GZMB Recombinant Antibody (CBLG1-295) (CAT#: CBMAB-G1012-LY)

Mouse Anti-GZMB Recombinant Antibody (CBLG1-294) (CAT#: CBMAB-G1011-LY)

Mouse Anti-GZMB Recombinant Antibody (CBLG1-293) (CAT#: CBMAB-G1010-LY)

Mouse Gzmb ELISA Kit (CAT#: V2LY-0626-LY878)

Human Recombinant GZMB protein, His Tag (CAT#: V2LY-0526-LY4406)

Isotype control

For research use only. Not intended for any clinical use.

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