Human Recombinant HECTD1 protein, GST Tag (V2LY-0526-LY4478)

Name: Human Recombinant HECTD1 protein, GST Tag
SKU: V2LY-0526-LY4478
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant HECTD1 protein, GST Tag consist of Amino Acid: 2129-2610 (end) and predicts a molecular mass of 75 kDa.

Molecule Mass

75 kDa

Sequence

Amino Acid: 2129-2610 (end)

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

HECT domain containing 1

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion. Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure.

Biological Process

Anatomical structure development Source: GO_Central
Aorta development Source: BHF-UCL
Heart valve development Source: BHF-UCL
Natural killer cell differentiation Source: Ensembl
Negative regulation of protein localization to plasma membrane Source: Ensembl
Neural tube closure Source: Ensembl
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
Protein autoubiquitination Source: Ensembl
Protein K63-linked ubiquitination Source: GO_Central
Spongiotrophoblast differentiation Source: Ensembl
Trophoblast giant cell differentiation Source: Ensembl
Ventricular septum development Source: BHF-UCL

Vaughan, N., Scholz, N., Lindon, C., & Licchesi, J. D. (2022). The E3 ubiquitin ligase HECTD1 contributes to cell proliferation through an effect on mitosis. Scientific Reports, 12(1), 13160.

Harris, L. D., Le Pen, J., Scholz, N., Mieszczanek, J., Vaughan, N., Davis, S., ... & Licchesi, J. D. (2021). The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1. Journal of Biological Chemistry, 296.

Dai, Q., Ma, Y., Xu, Z., Zhang, L., Yang, H., Liu, Q., & Wang, J. (2021). Downregulation of circular RNA HECTD1 induces neuroprotection against ischemic stroke through the microRNA-133b/TRAF3 pathway. Life Sciences, 264, 118626.

Chen, L., Luo, W., Zhang, W., Chu, H., Wang, J., Dai, X., ... & Chao, J. (2020). circDLPAG4/HECTD1 mediates ischaemia/reperfusion injury in endothelial cells via ER stress. RNA biology, 17(2), 240-253.

Wang, X., De Geyter, C., Jia, Z., Peng, Y., & Zhang, H. (2020). HECTD1 regulates the expression of SNAIL: Implications for epithelial‑mesenchymal transition. International Journal of Oncology, 56(5), 1186-1198.

Chu, H., Wang, W., Luo, W., Zhang, W., Cheng, Y., Huang, J., ... & Chao, J. (2019). CircHECTD1 mediates pulmonary fibroblast activation via HECTD1. Therapeutic Advances in Chronic Disease, 10, 2040622319891558.

Peng, X., Jing, P., Chen, J., & Xu, L. (2019). The role of circular RNA HECTD1 expression in disease risk, disease severity, inflammation, and recurrence of acute ischemic stroke. Journal of clinical laboratory analysis, 33(7), e22954.

Han, B., Zhang, Y., Zhang, Y., Bai, Y., Chen, X., Huang, R., ... & Yao, H. (2018). Novel insight into circular RNA HECTD1 in astrocyte activation via autophagy by targeting MIR142-TIPARP: implications for cerebral ischemic stroke. Autophagy, 14(7), 1164-1184.

Fang, S., Guo, H., Cheng, Y., Zhou, Z., Zhang, W., Han, B., ... & Chao, J. (2018). circHECTD1 promotes the silica-induced pulmonary endothelial–mesenchymal transition via HECTD1. Cell death & disease, 9(3), 396.

Zhou, Z., Jiang, R., Yang, X., Guo, H., Fang, S., Zhang, Y., ... & Chao, J. (2018). circRNA mediates silica-induced macrophage activation via HECTD1/ZC3H12A-dependent ubiquitination. Theranostics, 8(2), 575.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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