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Mouse Anti-HECTD1 Recombinant Antibody (1E10) (CBMAB-A3814-LY)

The product is antibody recognizes HECTD1. The antibody 1E10 immunoassay techniques such as: WB, ELISA.
See all HECTD1 antibodies
Published Data
Fig.1 HECTD1 protein was immunoprecipitated (IP) as indicated followed by immunoblotting (IB) with streptavidin (STV).
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Fig.2 Cells were starved overnight and plated on FN-coated slides for 60 min, followed by fixation and staining with HECTD1.
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Fig.3 Wildtype placentas were subjected to immunostaining with antibodies against Hectd1 along with Mct4.
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Fig.4 Immunohistochemistry using the anti-Hectd1 antibody.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1E10
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
HECTD1 (NP_056197, 3 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
HECT domain containing 1
Entrez Gene ID
25831
UniProt ID
Q9ULT8
Alternative Names
FLJ38315; KIAA1131
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion. Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure.
Biological Process
Anatomical structure development Source: GO_Central
Aorta development Source: BHF-UCL
Heart valve development Source: BHF-UCL
Natural killer cell differentiation Source: Ensembl
Negative regulation of protein localization to plasma membrane Source: Ensembl
Neural tube closure Source: Ensembl
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
Protein autoubiquitination Source: Ensembl
Protein K63-linked ubiquitination Source: GO_Central
Spongiotrophoblast differentiation Source: Ensembl
Trophoblast giant cell differentiation Source: Ensembl
Ventricular septum development Source: BHF-UCL

Vaughan, N., Scholz, N., Lindon, C., & Licchesi, J. D. (2022). The E3 ubiquitin ligase HECTD1 contributes to cell proliferation through an effect on mitosis. Scientific Reports, 12(1), 13160.

Harris, L. D., Le Pen, J., Scholz, N., Mieszczanek, J., Vaughan, N., Davis, S., ... & Licchesi, J. D. (2021). The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1. Journal of Biological Chemistry, 296.

Dai, Q., Ma, Y., Xu, Z., Zhang, L., Yang, H., Liu, Q., & Wang, J. (2021). Downregulation of circular RNA HECTD1 induces neuroprotection against ischemic stroke through the microRNA-133b/TRAF3 pathway. Life Sciences, 264, 118626.

Chen, L., Luo, W., Zhang, W., Chu, H., Wang, J., Dai, X., ... & Chao, J. (2020). circDLPAG4/HECTD1 mediates ischaemia/reperfusion injury in endothelial cells via ER stress. RNA biology, 17(2), 240-253.

Wang, X., De Geyter, C., Jia, Z., Peng, Y., & Zhang, H. (2020). HECTD1 regulates the expression of SNAIL: Implications for epithelial‑mesenchymal transition. International Journal of Oncology, 56(5), 1186-1198.

Chu, H., Wang, W., Luo, W., Zhang, W., Cheng, Y., Huang, J., ... & Chao, J. (2019). CircHECTD1 mediates pulmonary fibroblast activation via HECTD1. Therapeutic Advances in Chronic Disease, 10, 2040622319891558.

Peng, X., Jing, P., Chen, J., & Xu, L. (2019). The role of circular RNA HECTD1 expression in disease risk, disease severity, inflammation, and recurrence of acute ischemic stroke. Journal of clinical laboratory analysis, 33(7), e22954.

Han, B., Zhang, Y., Zhang, Y., Bai, Y., Chen, X., Huang, R., ... & Yao, H. (2018). Novel insight into circular RNA HECTD1 in astrocyte activation via autophagy by targeting MIR142-TIPARP: implications for cerebral ischemic stroke. Autophagy, 14(7), 1164-1184.

Fang, S., Guo, H., Cheng, Y., Zhou, Z., Zhang, W., Han, B., ... & Chao, J. (2018). circHECTD1 promotes the silica-induced pulmonary endothelial–mesenchymal transition via HECTD1. Cell death & disease, 9(3), 396.

Zhou, Z., Jiang, R., Yang, X., Guo, H., Fang, S., Zhang, Y., ... & Chao, J. (2018). circRNA mediates silica-induced macrophage activation via HECTD1/ZC3H12A-dependent ubiquitination. Theranostics, 8(2), 575.

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For research use only. Not intended for any clinical use.

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