Human Recombinant HGFA protein, His Tag (V2LY-0526-LY4544)

Name: Human Recombinant HGFA protein, His Tag
SKU: V2LY-0526-LY4544
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant HGFA protein, His Tag consist of Amino Acid: 1-655 and predicts a molecular mass of 68.2 kDa.

Molecule Mass

68.2 kDa

Sequence

Amino Acid: 1-655

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Hgf Activator

Function

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.

Biological Process

Blood coagulation Source: GO_Central
Proteolysis Source: GO_Central
Zymogen activation Source: GO_Central

Cellular Location

Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.

Sargsyan, A., Doridot, L., Hannou, S. A., Tong, W., Srinivasan, H., Ivison, R., ... & Herman, M. A. (2023). HGFAC is a ChREBP-regulated hepatokine that enhances glucose and lipid homeostasis. JCI insight, 8(1).

Banna, F. E., Otto, J. M., Mulloy, S. M., Tsai, W., McElroy, S. M., Wong, A. L., ... & Lee, A. M. (2022). Back-translating GWAS findings to animal models reveals a role for Hgfac and Slc39a8 in alcohol and nicotine consumption. Scientific reports, 12(1), 9336.

Sargsyan, A., Doridot, L., Hannou, S. A., Tong, W., Srinivasan, H., Ivison, R., ... & Herman, M. A. (2021). HGFAC is a ChREBP Regulated Hepatokine that Enhances Glucose and Lipid Homeostasis. bioRxiv, 2021-07.

Yin, L., Mu, Y., Lin, Y., & Xia, Q. (2019). HGFAC expression decreased in liver cancer and its low expression correlated with DNA hypermethylation and poor prognosis. Journal of Cellular Biochemistry, 120(6), 9692-9699.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human HGFAC ELISA Kit (CAT#: V2LY-0626-LY4662)

Mouse Hgfac ELISA Kit (CAT#: V2LY-0626-LY5878)

Related Products

Mouse Hgfac ELISA Kit (CAT#: V2LY-0626-LY5878)

Mouse Anti-HGFAC Recombinant Antibody (CBFYH-1036) (CAT#: CBMAB-H1981-FY)

Mouse Anti-HGFAC Recombinant Antibody (CBFYH-1037) (CAT#: CBMAB-H1982-FY)

Human HGFAC ELISA Kit (CAT#: V2LY-0626-LY4662)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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