Human Recombinant IFNAR2 protein, hFc Tag (V2LY-0526-LY5047)

Name: Human Recombinant IFNAR2 protein, hFc Tag
SKU: V2LY-0526-LY5047
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant IFNAR2 protein, hFc Tag consist of Amino Acid: 1-243 and predicts a molecular mass of 51.8 kDa.

Molecule Mass

51.8 kDa

Verified

HPLC

Sequence

Amino Acid: 1-243

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interferon Alpha And Beta Receptor Subunit 2

Function

Associates with IFNAR1 to form the plasma membrane receptor in the type I interferon signaling pathway (PubMed:26424569, PubMed:28165510, PubMed:32972995).

Directly involved in signal transduction through its association with the TYR kinase JAK1 (PubMed:8181059, PubMed:7665574, PubMed:7759950).

Involved in interferon-mediated STAT1, STAT2 and STAT3 activation (PubMed:26424569, PubMed:32972995).

Isoform 3:
Potent inhibitor of type I IFN receptor activity.

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
Cytokine-mediated signaling pathway Source: GO_Central
Defense response to virus Source: UniProtKB
Receptor signaling pathway via JAK-STAT Source: ProtInc
Response to interferon-alpha Source: UniProtKB
Response to interferon-beta Source: UniProtKB
Response to virus Source: ProtInc
Type I interferon signaling pathway Source: UniProtKB

Cellular Location

Isoform 1&2: Cell membrane
Isoform 3: Secreted

Involvement in disease

Immunodeficiency 45 (IMD45):
An autosomal recessive disorder characterized by increased susceptibility to viral infection due to impaired antiviral immunity, resulting in infection-associated encephalopathy. Affected individuals are at risk for developing fatal encephalitis after routine measles/mumps/rubella (MMR) vaccination.

Topology

Extracellular: 27-243
Helical: 244-264
Cytoplasmic: 265-515

PTM

Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types.
Glycosylated.

Duncan, C. J., Skouboe, M. K., Howarth, S., Hollensen, A. K., Chen, R., Børresen, M. L., ... & Mogensen, T. H. (2022). Life-threatening viral disease in a novel form of autosomal recessive IFNAR2 deficiency in the Arctic. Journal of Experimental Medicine, 219(6), e20212427.

Dieter, C., de Almeida Brondani, L., Lemos, N. E., Schaeffer, A. F., Zanotto, C., Ramos, D. T., ... & Crispim, D. (2022). Polymorphisms in ACE1, TMPRSS2, IFIH1, IFNAR2, and TYK2 genes are associated with worse clinical outcomes in COVID-19. Genes, 14(1), 29.

Fricke-Galindo, I., Martínez-Morales, A., Chávez-Galán, L., Ocaña-Guzmán, R., Buendía-Roldán, I., Pérez-Rubio, G., ... & Falfán-Valencia, R. (2022). IFNAR2 relevance in the clinical outcome of individuals with severe COVID-19. Frontiers in Immunology, 13, 949413.

Meyts, I. (2022). Null IFNAR1 and IFNAR2 alleles are surprisingly common in the Pacific and Arctic.

Zhang, L., Ma, J., Jin, X., Zhang, L., Zhang, M., Li, P. Z., ... & Zhang, L. (2022). Human IFNAR2 Mutant Generated by CRISPR/Cas9-Induced Exon Skipping Upregulates a Subset of Tonic-Like Interferon-Stimulated Genes Upon IFNβ Stimulation. Journal of Interferon & Cytokine Research, 42(11), 580-589.

Akter, S., Roy, A. S., Tonmoy, M. I. Q., & Islam, M. S. (2022). Deleterious single nucleotide polymorphisms (SNPs) of human IFNAR2 gene facilitate COVID-19 severity in patients: a comprehensive in silico approach. Journal of Biomolecular Structure and Dynamics, 40(21), 11173-11189.

Shemesh, M., Lochte, S., Piehler, J., & Schreiber, G. (2021). IFNAR1 and IFNAR2 play distinct roles in initiating type I interferon–induced JAK-STAT signaling and activating STATs. Science signaling, 14(710), eabe4627.

Smieszek, S. P., Polymeropoulos, V. M., Xiao, C., Polymeropoulos, C. M., & Polymeropoulos, M. H. (2021). Loss-of-function mutations in IFNAR2 in COVID-19 severe infection susceptibility. Journal of Global Antimicrobial Resistance, 26, 239-240.

Passarelli, C., Civino, A., Rossi, M. N., Cifaldi, L., Lanari, V., Moneta, G. M., ... & Prencipe, G. (2020). IFNAR2 deficiency causing dysregulation of NK cell functions and presenting with hemophagocytic lymphohistiocytosis. Frontiers in Genetics, 11, 937.

Shepardson, K. M., Larson, K., Johns, L. L., Stanek, K., Cho, H., Wellham, J., ... & Rynda-Apple, A. (2018). IFNAR2 is required for anti-influenza immunity and alters susceptibility to post-influenza bacterial superinfections. Frontiers in immunology, 9, 2589.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IFNAR2 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY5046)

Human Recombinant IFNAR2 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY5048)

Human Recombinant IFNAR2 protein, His Tag (CAT#: V2LY-0526-LY5049)

Cynomolgus Recombinant Interferon alpha/beta receptor 2 protein, ECD, His Tag (CAT#: V2LY-0526-LY1587)

Human IFNAR2 ELISA Kit (CAT#: V2LY-0626-LY4741)

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Human Recombinant IFNAR2 protein, His Tag (CAT#: V2LY-0526-LY5049)

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Human Recombinant IFNAR2 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY5048)

Human Recombinant IFNAR2 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY5046)

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Mouse Anti-IFNAR2 Recombinant Antibody (CBYY-I0715) (CAT#: CBMAB-I1594-YY)

Human IFNAR2 ELISA Kit (CAT#: V2LY-0626-LY4741)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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