Human Recombinant IL2 protein-1 (V2LY-0526-LY4827)

Name: Human Recombinant IL2 protein-1
SKU: V2LY-0526-LY4827
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Protein Construction

This product is Human Recombinant IL2 protein consist of Amino Acid: 21-153 and predicts a molecular mass of 15.4 kDa.

Molecule Mass

15.4 kDa

Verified

HPLC

Sequence

Amino Acid: 21-153

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.>95% as determined by SEC-HPLC.

Endotoxin

<10 EU/mg of the protein as determined by the LAL method

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interleukin 2

Alternative Names

Interleukin 2

Function

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Cellular Location

Secreted

Involvement in disease

A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

Liu, Y., Zhou, N., Zhou, L., Wang, J., Zhou, Y., Zhang, T., ... & Huang, B. (2021). IL-2 regulates tumor-reactive CD8+ T cell exhaustion by activating the aryl hydrocarbon receptor. Nature immunology, 22(3), 358-369.

Lopes, J. E., Fisher, J. L., Flick, H. L., Wang, C., Sun, L., Ernstoff, M. S., ... & Losey, H. C. (2020). ALKS 4230: a novel engineered IL-2 fusion protein with an improved cellular selectivity profile for cancer immunotherapy. Journal for immunotherapy of cancer, 8(1).

Damoiseaux, J. (2020). The IL-2–IL-2 receptor pathway in health and disease: The role of the soluble IL-2 receptor. Clinical Immunology, 218, 108515.

Papillion, A., Powell, M. D., Chisolm, D. A., Bachus, H., Fuller, M. J., Weinmann, A. S., ... & Ballesteros-Tato, A. (2019). Inhibition of IL-2 responsiveness by IL-6 is required for the generation of GC-TFH cells. Science immunology, 4(39), eaaw7636.

Forsberg, E. M., Lindberg, M. F., Jespersen, H., Alsén, S., Bagge, R. O., Donia, M., ... & Nilsson, J. A. (2019). HER2 CAR-T cells eradicate uveal melanoma and T-cell therapy–resistant human melanoma in IL2 transgenic NOD/SCID IL2 receptor knockout mice. Cancer Research, 79(5), 899-904.

Mortara, L., Balza, E., Bruno, A., Poggi, A., Orecchia, P., & Carnemolla, B. (2018). Anti-cancer therapies employing IL-2 cytokine tumor targeting: contribution of innate, adaptive and immunosuppressive cells in the anti-tumor efficacy. Frontiers in Immunology, 9, 2905.

Abbas, A. K., Trotta, E., R. Simeonov, D., Marson, A., & Bluestone, J. A. (2018). Revisiting IL-2: Biology and therapeutic prospects. Science immunology, 3(25), eaat1482.

Sockolosky, J. T., Trotta, E., Parisi, G., Picton, L., Su, L. L., Le, A. C., ... & Garcia, K. C. (2018). Selective targeting of engineered T cells using orthogonal IL-2 cytokine-receptor complexes. Science, 359(6379), 1037-1042.

Fan, M. Y., Low, J. S., Tanimine, N., Finn, K. K., Priyadharshini, B., Germana, S. K., ... & Turka, L. A. (2018). Differential roles of IL-2 signaling in developing versus mature Tregs. Cell reports, 25(5), 1204-1213.

DiToro, D., Winstead, C. J., Pham, D., Witte, S., Andargachew, R., Singer, J. R., ... & Weaver, C. T. (2018). Differential IL-2 expression defines developmental fates of follicular versus nonfollicular helper T cells. Science, 361(6407), eaao2933.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant IL2 (147 Cys/Ser) protein (CAT#: V2LY-0526-LY1277)

Human Recombinant IL2 protein, His Tag (CAT#: V2LY-0526-LY4824)

Human Recombinant IL2 protein (CAT#: V2LY-0526-LY4826)

Mouse Recombinant IL2 protein (CAT#: V2LY-0526-LY8512)

Mouse Recombinant IL2 protein, hFc Tag (CAT#: V2LY-0526-LY8513)

Rat Recombinant IL2 protein (CAT#: V2LY-0526-LY9454)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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