Human Recombinant IL36G protein, His Tag (V2LY-0526-LY4910)

Name: Human Recombinant IL36G protein, His Tag
SKU: V2LY-0526-LY4910
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant IL36G protein, His Tag consist of Amino Acid: 18-169 and predicts a molecular mass of 19.1 kDa.

Molecule Mass

19.1 kDa

Sequence

Amino Acid: 18-169

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interleukin 36 Gamma

Function

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.

Biological Process

Cell-cell signalingManual Assertion Based On ExperimentTAS:ProtInc
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIBA:GO_Central
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Inflammatory responseManual Assertion Based On ExperimentIBA:GO_Central
Inflammatory response to antigenic stimulusManual Assertion Based On ExperimentIBA:GO_Central
Innate immune responseIEA:UniProtKB-KW
Positive regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm; Secreted. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion.

PTM

N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold) (PubMed:21965679).
Proteolytically cleaved by cathepsin CTSG (PubMed:30804664).

Moreira, C. R., de Alcântara, C. C., Flauzino, T., Martin, L. M. M., Lozovoy, M. A. B., Reiche, E. M. V., & Simão, A. N. C. (2023). IL36G genetic variant is independently associated with susceptibility, severity and joint involvement in psoriasis. Molecular Immunology, 159, 69-75.

Merleev, A., Ji-Xu, A., Toussi, A., Tsoi, L. C., Le, S. T., Luxardi, G., ... & Maverakis, E. (2022). Proprotein convertase subtilisin/kexin type 9 is a psoriasis-susceptibility locus that is negatively related to IL36G. JCI insight, 7(16).

Lu, Y. W., Chen, Y. J., Shi, N., Yang, L. H., Wang, H. M., Dong, R. J., ... & Li, Y. Y. (2022). IL36G is associated with cutaneous antiviral competence in psoriasis. Frontiers in Immunology, 13, 971071.

Furue, K., Ito, T., Tanaka, Y., Yumine, A., Hashimoto-Hachiya, A., Takemura, M., ... & Furue, M. (2019). Cyto/chemokine profile of in vitro scratched keratinocyte model: Implications of significant upregulation of CCL20, CXCL8 and IL36G in Koebner phenomenon. Journal of dermatological science, 94(1), 244-251.

Traks, T., Keermann, M., Prans, E., Karelson, M., Loite, U., Kõks, G., ... & Kingo, K. (2019). Polymorphisms in IL36G gene are associated with plaque psoriasis. BMC Medical Genetics, 20, 1-8.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IL36G protein (CAT#: V2LY-0526-LY4908)

Human Recombinant IL36G protein-1 (CAT#: V2LY-0526-LY4909)

Mouse Recombinant IL36G protein, His Tag (CAT#: V2LY-0526-LY8541)

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Mouse Anti-IL36G Recombinant Antibody (2A8) (CAT#: CBMAB-A4376-LY)

Mouse Recombinant IL36G protein, His Tag (CAT#: V2LY-0526-LY8541)

Mouse Anti-IL36G Recombinant Antibody (2F4) (CAT#: CBMAB-I0563-YY)

Mouse Anti-IL36G Recombinant Antibody (CBYY-I1312) (CAT#: CBMAB-I2482-YY)

Human Recombinant IL36G protein (CAT#: V2LY-0526-LY4908)

Rat Anti-IL36G Recombinant Antibody (CBYY-I0212) (CAT#: CBMAB-I0562-YY)

Mouse Anti-IL36G Recombinant Antibody (CBYY-I1989) (CAT#: CBMAB-I3159-YY)

Mouse Anti-IL36G Recombinant Antibody (CBYY-I2118) (CAT#: CBMAB-I3288-YY)

Isotype control

For research use only. Not intended for any clinical use.

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