Human Recombinant ING5 protein, GST Tag (V2LY-0526-LY4997)

Name: Human Recombinant ING5 protein, GST Tag
SKU: V2LY-0526-LY4997
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant ING5 protein, GST Tag consist of Amino Acid: 36-84 and predicts a molecular mass of 32.9 kDa.

Molecule Mass

32.9 kDa

Sequence

Amino Acid: 36-84

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Inhibitor Of Growth Family Member 5

Function

Component of the HBO1 complex, which specifically mediates acetylation of histone H3 at 'Lys-14' (H3K14ac) and, to a lower extent, acetylation of histone H4 (PubMed:24065767).
Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity (PubMed:16387653).
Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator (PubMed:12750254, PubMed:16387653).
Inhibits cell growth, induces a delay in S-phase progression and enhances Fas-induced apoptosis in an INCA1-dependent manner (PubMed:21750715).

Biological Process

Chromatin organizationIEA:UniProtKB-KW
Biological Process DNA replicationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3 acetylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K14 acetylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H4 acetylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H4-K12 acetylationManual Assertion Based On ExperimentIDA:ComplexPortal
Histone H4-K5 acetylationManual Assertion Based On ExperimentIDA:ComplexPortal
Histone H4-K8 acetylationManual Assertion Based On ExperimentIDA:ComplexPortal
Histone modificationManual Assertion Based On ExperimentIDA:ComplexPortal
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of growthManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of apoptotic processManual Assertion Based On ExperimentIGI:MGI
Positive regulation of apoptotic signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Protein acetylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell cycleManual Assertion Based On ExperimentIDA:ComplexPortal
Regulation of cell growthManual Assertion Based On ExperimentIDA:ComplexPortal
Regulation of DNA biosynthetic processManual Assertion Based On ExperimentIDA:ComplexPortal
Regulation of DNA replicationManual Assertion Based On ExperimentIDA:ComplexPortal
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:ComplexPortal

Cellular Location

Nucleus; Chromosome. Localizes to transcription start sites.

Taheri, M., Hussen, B. M., Najafi, S., Abak, A., Ghafouri-Fard, S., Samsami, M., & Baniahmad, A. (2022). Molecular mechanisms of inhibitor of growth (ING) family members in health and malignancy. Cancer Cell International, 22(1), 272.

Ghafouri-Fard, S., Taheri, M., & Baniahmad, A. (2022). Inhibitor of growth factors regulate cellular senescence. Cancers, 14(13), 3107.

Xu, J. H., Zhao, J. X., Jiang, M. Y., Yang, L. P., Sun, M. L., & Wang, H. W. (2020). MiR-193 promotes cell proliferation and invasion by ING5/PI3K/AKT pathway of triple-negative breast cancer. European Review for Medical & Pharmacological Sciences, 24(6).

Xin, H., Wang, C., Chi, Y., & Liu, Z. (2020). MicroRNA-196b-5p promotes malignant progression of colorectal cancer by targeting ING5. Cancer Cell International, 20, 1-17.

Zhang, H., Zhou, J., Zhang, M., Yi, Y., & He, B. (2019). Upregulation of miR-376c-3p alleviates oxygen–glucose deprivation-induced cell injury by targeting ING5. Cellular & Molecular Biology Letters, 24(1), 1-12.

Gao, W., & Han, J. (2018). Overexpression of ING5 inhibits HGF-induced proliferation, invasion and EMT in thyroid cancer cells via regulation of the c-Met/PI3K/Akt signaling pathway. Biomedicine & Pharmacotherapy, 98, 265-270.

Zhang, X., Xu, Z. H., Xie, H., Sun, Y. W., Liu, J., & Zhao, Y. B. (2018). ING5 is a potential target for osteosarcoma therapy. Technology in Cancer Research & Treatment, 17, 1533033818762680.

Ye, P., Ke, X., Zang, X., Sun, H., Dong, Z., Lin, J., ... & Gao, L. (2018). Up-regulated MiR-27-3p promotes the G1-S phase transition by targeting inhibitor of growth family member 5 in osteosarcoma. Biomedicine & Pharmacotherapy, 101, 219-227.

Zhang, G. J., Zhao, J., Jiang, M. L., & Zhang, L. C. (2018). ING5 inhibits cell proliferation and invasion in esophageal squamous cell carcinoma through regulation of the Akt/NF-κB/MMP-9 signaling pathway. Biochemical and biophysical research communications, 496(2), 387-393.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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