Human Recombinant KLRG1 protein, ECD, hFc Tag (V2LY-0526-LY5197)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
hFc Tag
Protein Construction
This product is Human Recombinant KLRG1 protein, ECD, hFc Tag consist of Amino Acid: 60-195 and predicts a molecular mass of 42.31 kDa.
Molecule Mass
42.31 kDa
Protein Domain
ECD
Sequence
Amino Acid: 60-195
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥90% as determined by SDS-PAGE.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Killer Cell Lectin Like Receptor G1
Function
Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.
Biological Process
Cell surface receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Cellular defense responseManual Assertion Based On ExperimentTAS:ProtInc
Inflammatory responseManual Assertion Based On ExperimentTAS:ProtInc
Innate immune responseIEA:UniProtKB-KW
Cellular Location
Cell membrane
Topology
Cytoplasmic: 1-38
Helical: 39-59
Extracellular: 60-195

Assatova, B., Trevisani, C., Willim, R., Duffy, J., Louissaint, A., Kim, W. S., ... & Jain, S. (2022). KLRG1 Depletion Is a Novel Therapeutic Strategy for Patients with Mature T and NK/T-Cell Lymphomas. Blood, 140(Supplement 1), 2253-2255.

Bag, A. K., Schultz, A. R., Goala, P., Karreth, F. A., & Adeegbe, D. O. (2021). Investigating the role of KLRG1 in regulatory T-cells and implications for anti-tumor immunity in Non-small Cell Lung Cancer. The Journal of Immunology, 206(1_Supplement), 57-02.

Yang, X., Zheng, Y., Han, Z., & Zhang, X. (2021). Functions and clinical significance of KLRG1 in the development of lung adenocarcinoma and immunotherapy. BMC cancer, 21, 1-16.

Tata, A., Dodard, G., Fugère, C., Leget, C., Ors, M., Rossi, B., ... & Brossay, L. (2021). Combination blockade of KLRG1 and PD-1 promotes immune control of local and disseminated cancers. Oncoimmunology, 10(1), 1933808.

Bottois, H., Ngollo, M., Hammoudi, N., Courau, T., Bonnereau, J., Chardiny, V., ... & Le Bourhis, L. (2020). Klrg1 and CD103 expressions define distinct intestinal tissue-resident memory CD8 T cell subsets modulated in Crohn's disease. Frontiers in Immunology, 11, 896.

Bull, N. C., Stylianou, E., Kaveh, D. A., Pinpathomrat, N., Pasricha, J., Harrington-Kandt, R., ... & McShane, H. (2019). Enhanced protection conferred by mucosal BCG vaccination associates with presence of antigen-specific lung tissue-resident PD-1+ KLRG1− CD4+ T cells. Mucosal immunology, 12(2), 555-564.

Remmerswaal, E. B., Hombrink, P., Nota, B., Pircher, H., Ten Berge, I. J., van Lier, R. A., & van Aalderen, M. C. (2019). Expression of IL‐7Rα and KLRG1 defines functionally distinct CD8+ T‐cell populations in humans. European Journal of Immunology, 49(5), 694-708.

Herndler-Brandstetter, D., Ishigame, H., Shinnakasu, R., Plajer, V., Stecher, C., Zhao, J., ... & Flavell, R. A. (2018). KLRG1+ effector CD8+ T cells lose KLRG1, differentiate into all memory T cell lineages, and convey enhanced protective immunity. Immunity, 48(4), 716-729.

Hu, Z., Zhao, H. M., Li, C. L., Liu, X. H., Barkan, D., Lowrie, D. B., ... & Fan, X. Y. (2018). The role of KLRG1 in human CD4+ T-cell immunity against tuberculosis. The Journal of Infectious Diseases, 217(9), 1491-1503.

Kadel, S., Ainsua-Enrich, E., Hatipoglu, I., Turner, S., Singh, S., Khan, S., & Kovats, S. (2018). A major population of functional KLRG1–ILC2s in female lungs contributes to a sex bias in ILC2 numbers. Immunohorizons, 2(2), 74-86.

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For research use only. Not intended for any clinical use.

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