Human Recombinant LILRB3 protein, ECD, Biotin Conjugated, His Tag (V2LY-0526-LY4989)

Name: Human Recombinant LILRB3 protein, ECD, Biotin Conjugated, His Tag
SKU: V2LY-0526-LY4989
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant LILRB3 protein, ECD, Biotin Conjugated, His Tag consist of Amino Acid: 1-443 and predicts a molecular mass of 47.6 kDa.

Molecule Mass

47.6 kDa

Protein Domain

ECD

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-443

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

leukocyte immunoglobulin like receptor B3

Function

May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
Cell surface receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Defense responseManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of osteoclast differentiationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 24-443
Helical: 444-464
Cytoplasmic: 465-631

PTM

Phosphorylated on tyrosine residues by LYN. Phosphorylation at Tyr-595 and Tyr-625 is important for interaction with PTPN6/SHP-1 and PTPN11/SHP-2.

Huang, R., Liu, X., Kim, J., Deng, H., Deng, M., Gui, X., ... & Zhang, C. (2023). LILRB3 supports immunosuppressive activity of myeloid cells and tumor development. Cancer Immunology Research.

Mai, S., Hodges, A., Chen, H. M., Zhang, J., Wang, Y. L., Liu, Y., ... & Pan, P. Y. (2023). LILRB3 Modulates Acute Myeloid Leukemia Progression and Acts as an Effective Target for CAR T-cell Therapy. Cancer Research, 83(24), 4047-4062.

Zhou, J., Wang, Y., Huang, G., Yang, M., Zhu, Y., Jin, C., ... & Shi, Y. (2023). LilrB3 is a putative cell surface receptor of APOE4. Cell Research, 33(2), 116-130.

Zhuang, Q., Liu, Y., Wang, H., Lin, Z., Sun, L., Liu, Y., ... & Mao, Y. (2023). LILRB3 suppresses immunity in glioma and is associated with poor prognosis. Clinical and Translational Medicine, 13(10).

Bashirova, A. A., Kasprzak, W., O’hUigin, C., & Carrington, M. (2023). Distinct frequency patterns of LILRB3 and LILRA6 allelic variants in Europeans. Immunogenetics, 75(3), 263-267.

Wu, G., Xu, Y., Schultz, R. D., Chen, H., Xie, J., Deng, M., ... & Zhang, C. C. (2021). LILRB3 supports acute myeloid leukemia development and regulates T-cell antitumor immune responses through the TRAF2–cFLIP–NF-κB signaling axis. Nature cancer, 2(11), 1170-1184.

Ayukawa, S., Kamoshita, N., Nakayama, J., Teramoto, R., Pishesha, N., Ohba, K., ... & Maruyama, T. (2021). Epithelial cells remove precancerous cells by cell competition via MHC class I–LILRB3 interaction. Nature Immunology, 22(11), 1391-1402.

Hirayasu, K., Sun, J., Hasegawa, G., Hashikawa, Y., Hosomichi, K., Tajima, A., ... & Hanayama, R. (2021). Characterization of LILRB3 and LILRA6 allelic variants in the Japanese population. Journal of Human Genetics, 66(7), 739-748.

Yeboah, M., Papagregoriou, C., Jones, D. C., Chan, H. C., Hu, G., McPartlan, J. S., ... & Roghanian, A. (2020). LILRB3 (ILT5) is a myeloid cell checkpoint that elicits profound immunomodulation. JCI insight, 5(18).

Zhao, Y., van Woudenbergh, E., Zhu, J., Heck, A. J., van Kessel, K. P., de Haas, C. J., ... & McCarthy, A. J. (2020). The orphan immune receptor LILRB3 modulates fc receptor–mediated functions of neutrophils. The Journal of Immunology, 204(4), 954-966.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant LILRB3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY4987)

Human Recombinant LILRB3 protein, ECD, His Tag (CAT#: V2LY-0526-LY4988)

Mouse Recombinant LILRB3 protein, His Tag (CAT#: V2LY-0526-LY8558)

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Isotype control

For research use only. Not intended for any clinical use.

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